Evidence map›Paper›PMID 25195016›Full record

ArticleNuclear medicine and biology

Inflammation imaging of atherosclerosis in Apo-E-deficient mice using a (99m)Tc-labeled dual-domain cytokine ligand.

Zhonglin Liu, Lilach O Lerman, Hui Tang, Christy Barber, Li Wan, Mizhou M Hui, Lars R Furenlid, James M Woolfenden

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nuclear medicine and biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Phenotypic transformation of intimal and adventitial lymphatics in atherosclerosis: a regulatory role for soluble VEGF receptor 2.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Zhonglin LiuDepartment of Medical Imaging, University of Arizona, Tucson, AZ, USA. Electronic address: zliu@radiology.arizona.edu.
Lilach O LermanDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, USA.
Hui TangDivision of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, USA.
Christy BarberDepartment of Medical Imaging, University of Arizona, Tucson, AZ, USA.
Li WanDepartment of Medical Imaging, University of Arizona, Tucson, AZ, USA.
Mizhou M HuiAmProtein Cooperation, San Gabriel, CA, USA.
Lars R FurenlidDepartment of Medical Imaging, University of Arizona, Tucson, AZ, USA.
James M WoolfendenDepartment of Medical Imaging, University of Arizona, Tucson, AZ, USA.
Banner - University Medical Center Tucson · USMayo Clinic · USPollinator Partnership · US

Funding

VITAMIN AP30CA023074 · NCI · UNIVERSITY OF ARIZONA · PI Dan Theodorescu · 1985 to 2026
$110.2M
CENTER FOR GAMMA RAY IMAGINGP41EB002035 · NIBIB · UNIVERSITY OF ARIZONA · PI BARRETT, HARRISON H, FURENLID, LARS R · 2003 to 2018
$17.7M
Tc-99m-Labled Cytokine Ligands and SPECT Inflammation Imaging of Ischemic HeartsR01HL090716 · NHLBI · UNIVERSITY OF ARIZONA · PI LIU, ZHONGLIN · 2009 to 2012
$1.5M
NCI NIH HHS P30 CA023074NHLBI NIH HHS R01 HL090716NHLBI NIH HHS R01-HL090716NIBIB NIH HHS P41 EB002035NIBIB NIH HHS P41-EB002035
6 · The paper itself

Abstract

unlabelledInterleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) play a critical role in initiating and accelerating atherosclerosis. This study evaluated the imaging properties of (99m)Tc-TNFR2-Fc-IL-1RA ((99m)Tc-TFI), a dual-domain cytokine radioligand that targets TNF-α and IL-1β pathways, in assessing atherosclerosis development in apolipoprotein-E-deficient (ApoE(-)(/)(-)) mice.

methodsThe feasibility and specificity of detecting atherosclerosis with (99m)Tc-TFI SPECT imaging were investigated in ApoE(-)(/)(-) and ApoE(+)(/)(+) mice. Fifty-four ApoE(-)(/)(-) mice were fed either an atherogenic diet (AGD) or a normal diet (ND) beginning at 5 weeks of age. Eighteen Apo-E wild-type (ApoE(+)(/)(+)) mice were fed an ND. Two groups of ApoE(-)(/)(-) mice (n=12 each group) on AGD and ND were imaged three times with (99m)Tc-TFI and a high-resolution SPECT system at 20-25, 30-40, and 48-52 weeks to study the evolution of atherosclerotic plaque.

resultsFocal radioactive accumulations in the aortic arch region were observed in the ApoE(-)(/)(-) mice (n=12) on AGD but not in the ApoE(+)(/)(+) mice on ND (n=10). Apo-E(-)(/)(-) mice on ND (n=11) exhibited lower radioactive uptake than ApoE(-)(/)(-) mice on AGD (P<0.05). Co-injection of an excess of cold ligand with (99m)Tc-TFI resulted in significant reduction of (99m)Tc-TFI uptake in the ApoE(-)(/)(-) mice on AGD. Longitudinal studies showed that (99m)Tc-TFI uptake in the aortas of ApoE(-)(/)(-) mice progressively increased from 20 to 48 weeks. Real-time PCR assays demonstrated that atherosclerotic aortas expressed significantly higher IL-1β and TNF-α than the aortas from wild-type controls.

conclusionsAtherosclerotic plaques were detected by (99m)Tc-TFI imaging in ApoE(-)(/)(-) mice. (99m)Tc-TFI is promising for specific detection of inflammatory response in atherosclerotic plaques.

Indexed as

Organotechnetium CompoundsAnimalsApolipoproteins EAtherosclerosisFemaleImage Processing, Computer-AssistedInflammationInterleukin-1betaInterleukin 1 Receptor Antagonist ProteinMaleMiceMice, Inbred C57BLMice, KnockoutMolecular ImagingReceptors, Tumor Necrosis Factor, Type IITomography, Emission-Computed, Single-PhotonApolipoproteins EInterleukin-1betaInterleukin 1 Receptor Antagonist ProteinOrganotechnetium CompoundsReceptors, Tumor Necrosis Factor, Type IITumor Necrosis Factor-alphaApolipoprotein-E-deficient miceAtherosclerosisInflammationInterleukin-1SPECTTumor necrosis factor

Identifiers

PMID25195016
PMCPMC4192104
OpenAlexW2014451680

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.