Evidence map›Paper›PMID 25194930›Full record

ArticleActa biomaterialia2014

Retinal ganglion cell polarization using immobilized guidance cues on a tissue-engineered scaffold.

Karl E Kador, Haneen S Alsehli, Allison N Zindell, Lung W Lau, Fotios M Andreopoulos, Brant D Watson, Jeffrey L Goldberg

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 56 citations in OpenAlex.

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  8. Bioengineering strategies for restoring vision.Nature biomedical engineering · 2023
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  14. Frontiers in cell and developmental biology · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Karl E KadorShiley Eye Center and Institute of Engineering in Medicine, University of California San Diego, La Jolla, CA 92093, USA; Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, Miller School of Medicine, University of Miami, FL 33136, USA.
Haneen S AlsehliBascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, Miller School of Medicine, University of Miami, FL 33136, USA; Department of Biomedical Sciences, Barry University, Miami Shores, FL 33161, USA.
Allison N ZindellBascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, Miller School of Medicine, University of Miami, FL 33136, USA; Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33136, USA.
Lung W LauBascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, Miller School of Medicine, University of Miami, FL 33136, USA.
Fotios M AndreopoulosDepartment of Biomedical Engineering, University of Miami, Coral Gables, FL 33136, USA; Department of Surgery, Miller School of Medicine, University of Miami, FL 33136, USA.
Brant D WatsonDepartment of Biomedical Engineering, University of Miami, Coral Gables, FL 33136, USA; Department of Neurology, Miller School of Medicine, University of Miami, FL 33136, USA.
Jeffrey L GoldbergShiley Eye Center and Institute of Engineering in Medicine, University of California San Diego, La Jolla, CA 92093, USA; Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, Miller School of Medicine, University of Miami, FL 33136, USA. Electronic address: jlgoldberg@ucsd.edu.
University of Miami · USUniversity of California, San Diego · US

Funding

Shared Equipment ModuleP30EY014801 · NEI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI Victor L Perez · 2004 to 2026
$12.2M
Vision BiostatisticsP30EY022589 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Derek Stuart Welsbie · 2012 to 2026
$10.3M
Retinal Scaffolds: Synaptic and Stem Cell IntegrationRC1EY020297 · NEI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI GOLDBERG, JEFFREY L · 2009 to 2010
$763k
NEI NIH HHS P30 EY014801NEI NIH HHS P30EY014801NEI NIH HHS P30 EY022589NEI NIH HHS P30-EY022589NEI NIH HHS RC1 EY020297NEI NIH HHS RC1-EY020297
6 · The paper itself

Abstract

Cell transplantation therapies to treat diseases related to dysfunction of retinal ganglion cells (RGCs) are limited in part by an inability to navigate to the optic nerve head within the retina. During development, RGCs are guided by a series of neurotrophic factors and guidance cues; however, these factors and their receptors on the RGCs are developmentally regulated and often not expressed during adulthood. Netrin-1 is a guidance factor capable of guiding RGCs in culture and relevant to guiding RGC axons toward the optic nerve head in vivo. Here we immobilized Netrin-1 using UV-initiated crosslinking to form a gradient capable of guiding the axonal growth of RGCs on a radial electrospun scaffold. Netrin-gradient scaffolds promoted both the percentage of RGCs polarized with a single axon, and also the percentage of cells polarized toward the scaffold center, from 31% to 52%. Thus, an immobilized protein gradient on a radial electrospun scaffold increases RGC axon growth in a direction consistent with developmental optic nerve head guidance, and may prove beneficial for use in cell transplant therapies for the treatment of glaucoma and other optic neuropathies.

Indexed as

Tissue ScaffoldsAdsorptionAnimalsAnimals, NewbornAxonsCell PolarityCell ProliferationCells, CulturedCoated Materials, BiocompatibleEquipment DesignEquipment Failure AnalysisGuided Tissue RegenerationMiceNerve Growth FactorsNetrin-1Retinal Ganglion CellsCoated Materials, BiocompatibleNerve Growth FactorsNetrin-1Ntn1 protein, mouseTumor Suppressor ProteinsAxon guidanceNetrinProtein immobilizationRetinal ganglion cell

Identifiers

PMID25194930
PMCPMC4254021
OpenAlexW2071816636

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.