ArticlePlant molecular biology2014
A Brassica napus PHT1 phosphate transporter, BnPht1;4, promotes phosphate uptake and affects roots architecture of transgenic Arabidopsis.
Article in Plant molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 49 citations in OpenAlex.
- Cross-kingdom sRNA Ta_sRNA1 silences PRIM2 to fine-tune Arabidopsis immunity during symbiosis with Trichoderma atroviride.The New phytologist · 2026Article
- From genes to traits: maximizing phosphorus utilization efficiency in crop plants.Frontiers in plant science · 2025Review
- Characterizing the wheat (Frontiers in plant science · 2025Article
- Multiomics dissection of Brassica napus L. lateral roots and endophytes interactions under phosphorus starvation.Nature communications · 2024Article
- Identification of GmPT proteins and investigation of their expressions in response to abiotic stress in soybean.Planta · 2024Article
- Comprehensive Evolution and Expression anaLysis of PHOSPHATE 1 Gene Family in Allotetraploid Brassica napus and Its Diploid Ancestors.Biochemical genetics · 2023Article
- Isolation and Characterization ofPlants (Basel, Switzerland) · 2023Article
- Phosphate TransporterPlants (Basel, Switzerland) · 2023Article
- Effects of Soybean Phosphate Transporter GeneInternational journal of molecular sciences · 2023Article
- Integrating genome-wide association studies with selective sweep reveals genetic loci associated with tolerance to low phosphate availability inMolecular breeding : new strategies in plant improvement · 2023Article
- Five Post-Translational Modification Residues of CmPT2 Play Key Roles in Yeast and Rice.International journal of molecular sciences · 2023Article
- Genome-Scale Investigation ofInternational journal of molecular sciences · 2022Article
- Overexpression of a Eutrema salsugineum phosphate transporter gene EsPHT1;4 enhances tolerance to low phosphorus stress in soybean.Biotechnology letters · 2020Article
- Genome-Wide Identification and Expression Profile Analysis of theInternational journal of molecular sciences · 2020Article
- Global Survey and Expressions of the Phosphate Transporter Gene Families inInternational journal of molecular sciences · 2020Article
- Functional Analysis of the Phosphate Transporter GeneFrontiers in plant science · 2020Article
- Engineering Multiple Abiotic Stress Tolerance in Canola,Frontiers in plant science · 2020Review
- Two-factor ANOVA of SSH and RNA-seq analysis reveal development-associated Pi-starvation genes in oilseed rape.Planta · 2019Article
- The high-affinity transporter BnPHT1;4 is involved in phosphorus acquisition and mobilization for facilitating seed germination and early seedling growth of Brassica napus.BMC plant biology · 2019Article
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8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localization and 12 transmembrane-spanning domains. Quantitative reverse-transcription PCR analysis and promoter activity assay showed BnPht1;4 was inert in plants under Pi sufficient conditions. However, expression of this gene was remarkably enhanced in roots under Pi deficient conditions. Interestingly, under low Pi conditions, its promoter activity is impaired in tips of elongated roots, suggesting that the high-affinity Pi transporter may be not involved in low Pi response at root tip area. The experimental results also indicated that BnPht1;4 induction by Pi deficiency is dependent on the existence of sugar. In 35S:BnPht1;4 transgenic Arabidopsis, the increase of Pi availability resulted in the change of root architecture under Pi deficient conditions, showing longer primary roots and lower lateral root density than that of wild type. By cis-element analysis, two P1BS and two W-box elements were found in BnPht1;4 promoter. Yeast one-hybrid assay indicated that PHR1 could bind to the BnPht1;4 promoter. P1BS elements in BnPht1;4 promoter are essential for BnPht1;4 induction in Pi starvation response. Furthermore, WRKY75 could bind to the BnPht1;4 promoter, in which W-box elements are important for this binding. These results indicated BnPht1;4 may be dually controlled by two family regulators under low Pi responses. Thus, our data on the regulative mechanism of high-affinity Pi transporter in Pi starvation response will be valuable for B. napus molecular agriculture.
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