ArticleApplied and environmental microbiology2014
Genetic characterization and comparison of Clostridium botulinum isolates from botulism cases in Japan between 2006 and 2011.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
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- Human and animal botulism surveillance in France from 2008 to 2019.Frontiers in public health · 2022Article
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- The Distinctive Evolution ofFrontiers in microbiology · 2021Article
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- Genomic Characterization of Newly Completed Genomes of Botulinum Neurotoxin-Producing Species from Argentina, Australia, and Africa.Genome biology and evolution · 2020Article
- ClosingFrontiers in microbiology · 2020Article
- Variations in the Botulinum Neurotoxin Binding Domain and the Potential for Novel Therapeutics.Toxins · 2018Review
- Differentiating Botulinum Neurotoxin-Producing Clostridia with a Simple, Multiplex PCR Assay.Applied and environmental microbiology · 2017Article
- Pulsotype Diversity of Clostridium botulinum Strains Containing Serotypes A and/or B Genes.Foodborne pathogens and disease · 2017Article
- Genetic Characterization of the Exceptionally High Heat Resistance of the Non-toxic SurrogateFrontiers in microbiology · 2017Article
- Impact ofCurrent opinion in food science · 2016Review
- Diversity of Group I and II Clostridium botulinum Strains from France Including Recently Identified Subtypes.Genome biology and evolution · 2016Article
- Comparative genomic analyses reveal broad diversity in botulinum-toxin-producing Clostridia.BMC genomics · 2016Article
- Genomic Epidemiology of Clostridium botulinum Isolates from Temporally Related Cases of Infant Botulism in New South Wales, Australia.Journal of clinical microbiology · 2015Article
- Implications of Genome-Based Discrimination between Clostridium botulinum Group I and Clostridium sporogenes Strains for Bacterial Taxonomy.Applied and environmental microbiology · 2015Article
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Authors and funding
10 authors.
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Abstract
Genetic characterization was performed for 10 group I Clostridium botulinum strains isolated from botulism cases in Japan between 2006 and 2011. Of these, 1 was type A, 2 were type B, and 7 were type A(B) {carrying a silent bont/B [bont/(B)] gene} serotype strains, based on botulinum neurotoxin (BoNT) production. The type A strain harbored the subtype A1 BoNT gene (bont/A1), which is associated with the ha gene cluster. The type B strains carried bont/B5 or bont/B6 subtype genes. The type A(B) strains carried bont/A1 identical to that of type A(B) strain NCTC2916. However, bont/(B) genes in these strains showed single-nucleotide polymorphisms (SNPs) among strains. SNPs at 2 nucleotide positions of bont/(B) enabled classification of the type A(B) strains into 3 groups. Pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem-repeat analysis (MLVA) also provided consistent separation results. In addition, the type A(B) strains were separated into 2 lineages based on their plasmid profiles. One lineage carried a small plasmid (5.9 kb), and another harbored 21-kb plasmids. To obtain more detailed genetic information about the 10 strains, we sequenced their genomes and compared them with 13 group I C. botulinum genomes in a database using whole-genome SNP analysis. This analysis provided high-resolution strain discrimination and enabled us to generate a refined phylogenetic tree that provides effective traceability of botulism cases, as well as bioterrorism materials. In the phylogenetic tree, the subtype B6 strains, Okayama2011 and Osaka05, were distantly separated from the other strains, indicating genomic divergence of subtype B6 strains among group I strains.
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