ArticleThe Journal of membrane biology2014
High-resolution structure of a membrane protein transferred from amphipol to a lipidic mesophase.
Article in The Journal of membrane biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Crystallographic insights into lipid-membrane protein interactions in microbial rhodopsins.Frontiers in molecular biosciences · 2024Article
- Mechanisms of Formation, Structure, and Dynamics of Lipoprotein Discs Stabilized by Amphiphilic Copolymers: A Comprehensive Review.Nanomaterials (Basel, Switzerland) · 2022Review
- Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures.Journal of applied crystallography · 2020Article
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Solution NMR: A powerful tool for structural and functional studies of membrane proteins in reconstituted environments.The Journal of biological chemistry · 2019Review
- Thermal melt circular dichroism spectroscopic studies for identifying stabilising amphipathic molecules for the voltage-gated sodium channel NavMs.Biopolymers · 2018Article
- Efficient non-cytotoxic fluorescent staining of halophiles.Scientific reports · 2018Article
- Light-independent phospholipid scramblase activity of bacteriorhodopsin from Halobacterium salinarum.Scientific reports · 2017Article
- Chemically Stable Lipids for Membrane Protein Crystallization.Crystal growth & design · 2017Article
- Large-scale production and protein engineering of G protein-coupled receptors for structural studies.Frontiers in pharmacology · 2015Review
- Membrane proteins, detergents and crystals: what is the state of the art?Acta crystallographica. Section F, Structural biology communications · 2014Review
- Amphipols for each season.The Journal of membrane biology · 2014Review
- Functionalized amphipols: a versatile toolbox suitable for applications of membrane proteins in synthetic biology.The Journal of membrane biology · 2014Review
- Amphipols and photosynthetic light-harvesting pigment-protein complexes.The Journal of membrane biology · 2014Article
- Solution behavior and crystallization of cytochrome bc₁ in the presence of amphipols.The Journal of membrane biology · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 8 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amphipols (APols) have become important tools for the stabilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs solubilized in APols would be of high importance for structural biology. However, despite considerable efforts, it is still not clear whether MP/APol complexes can form well-ordered crystals suitable for X-ray crystallography. In the present work, we show that an APol-trapped MP can be crystallized in meso. Bacteriorhodopsin (BR) trapped by APol A8-35 was mixed with a lipidic mesophase, and crystallization was induced by adding a precipitant. The crystals diffract beyond 2 Å. The structure of BR was solved to 2 Å and found to be indistinguishable from previous structures obtained after transfer from detergent solutions. We suggest the proposed protocol of in meso crystallization to be generally applicable to APol-trapped MPs.
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