Evidence map›Paper›PMID 25192941›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2014

Delivery of antisense oligonucleotides using poly(alkylene oxide)-poly(propylacrylic acid) graft copolymers in conjunction with cationic liposomes.

Lavanya Y Peddada, Olga B Garbuzenko, David I Devore, Tamara Minko, Charles M Roth

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
  2. Antimicrobial Loaded Graft-Copolymer Nanoparticles for Treatment ofbioRxiv : the preprint server for biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Lavanya Y PeddadaDepartment of Biomedical Engineering, Rutgers University, Piscataway, USA.
Olga B GarbuzenkoDepartment of Pharmaceutics, Rutgers University, Piscataway, USA.
David I DevoreU.S. Army Institute of Surgical Research, Fort Sam Houston, 78234, USA.
Tamara MinkoDepartment of Pharmaceutics, Rutgers University, Piscataway, USA.
Charles M RothDepartment of Biomedical Engineering, Rutgers University, Piscataway, USA; Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, USA. Electronic address: cmroth@rutgers.edu.
Rutgers, The State University of New Jersey · USUnited States Army Institute of Surgical Research · US

Funding

Efficient Cellular Delivery of OligonucleotidesR01EB008278 · NIBIB · RUTGERS, THE STATE UNIV OF N.J. · PI ROTH, CHARLES M. · 2009 to 2010
$688k
NIBIB NIH HHS 2R01EB008278NIBIB NIH HHS R01 EB008278
6 · The paper itself

Abstract

The clinical application of gene silencing is hindered by poor stability and low delivery efficiency of naked oligonucleotides. Here, we present the in vitro and in vivo behaviors of a rationally designed, ternary, self-assembled nanoparticle complex, consisting of an anionic copolymer, cationic DOTAP liposome, and antisense oligonucleotide (AON). The multifunctional copolymers are based on backbone poly(propylacrylic acid) (PPAA), a pH-sensitive hydrophobic polymer, with grafted poly(alkylene oxides) (PAOs) varying in extent of grafting and PAO chemistry. The nanoparticle complexes with PPAA-g-PAO copolymers enhance antisense gene silencing effects in A2780 human ovarian cancer cells. A greater amount of AON is delivered to ovarian tumor xenografts using the ternary copolymer-stabilized delivery system, compared to a binary DOTAP/AON complex, following intraperitoneal injection in mice. Further, intratumoral injection of the nanoparticle complexes containing 1 mol% grafted PAO reduced tumoral bcl-2 expression by up to 60%. The data for complexes across the set of PAO polymers support a strong role for the hydrophilic-lipophilic balance of the graft copolymer in achieving serum stability and cellular uptake. Based upon these results, we anticipate that this novel nanoparticle delivery system can be extended to the delivery of plasmid DNA, siRNA, or aptamers for preclinical and clinical development.

Indexed as

1,2-DipalmitoylphosphatidylcholineAcrylamidesAcrylic ResinsAnimalsCationsCell Line, TumorDrug CarriersDrug Delivery SystemsFemaleGenes, bcl-2Gene SilencingGenetic TherapyHemolysisHumansLiposomesMice1,2-DipalmitoylphosphatidylcholineAcrylamidesAcrylic ResinsCationsDrug CarriersLiposomesOligonucleotides, AntisensePolyethylene Glycolspoly(N-isopropylacrylamide-co-propylacrylic acid)AntisenseDrug deliveryGene silencingNanoparticleSelf assemblyTumor

Identifiers

PMID25192941
PMCPMC4573985
OpenAlexW2045910740

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.