ArticlePlant physiology2014
Broad 4-hydroxyphenylpyruvate dioxygenase inhibitor herbicide tolerance in soybean with an optimized enzyme and expression cassette.
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Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
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Who cites it
30 citing papers in PubMed, 81 citations in OpenAlex.
- A century of vitamin E research: The innovative journey from basic biology to synthetic bio-manufacturing.Journal of integrative plant biology · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Identification and application of herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase (HPPD) alleles via directed evolution.Plant biotechnology journal · 2025Article
- Characterization of a Topramezone-Resistant Rice Mutant TZR1: Insights into GST-Mediated Detoxification and Antioxidant Responses.Plants (Basel, Switzerland) · 2025Article
- Identifying resistant mutations in the herbicide target site of the plant 4-hydroxyphenylpyruvate dioxygenase.Plant biotechnology journal · 2025Article
- Article
- Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean.Pest management science · 2022Article
- Development of an efficient marker-free soybean transformation method using the novel bacterium Ochrobactrum haywardense H1.Plant biotechnology journal · 2022Article
- Vitamin E synthesis and response in plants.Frontiers in plant science · 2022Review
- Mechanism of action and selectivity of a novel herbicide, fenquinotrione.Journal of pesticide science · 2021Article
- Based on the Virtual Screening of Multiple Pharmacophores, Docking and Molecular Dynamics Simulation Approaches toward the Discovery of Novel HPPD Inhibitors.International journal of molecular sciences · 2020Article
- Role of cytosolic, tyrosine-insensitive prephenate dehydrogenase inPlant direct · 2020Article
- Characterization, Genetic Analyses, and Identification of QTLs Conferring Metabolic Resistance to a 4-Hydroxyphenylpyruvate Dioxygenase Inhibitor in Sorghum (Frontiers in plant science · 2020Article
- Transgenic crops for the agricultural improvement in Pakistan: a perspective of environmental stresses and the current status of genetically modified crops.GM crops & food · 2020Review
- Isolation and characterization of a novel bacterial strain from a Tris-Acetate-Phosphate agar medium plate of the green micro-algaF1000Research · 2020Article
- A robust bacterial assay for high-throughput screening of human 4-hydroxyphenylpyruvate dioxygenase inhibitors.Scientific reports · 2019Article
- Free energy calculations elucidate substrate binding, gating mechanism, and tolerance-promoting mutations in herbicide target 4-hydroxyphenylpyruvate dioxygenase.Protein science : a publication of the Protein Society · 2019Article
- TAT1 and TAT2 tyrosine aminotransferases have both distinct and shared functions in tyrosine metabolism and degradation inThe Journal of biological chemistry · 2019Article
- Evidence for photolytic and microbial degradation processes in the dissipation of leptospermone, a natural β-triketone herbicide.Environmental science and pollution research international · 2018Article
- Combination of Virtual Screening Protocol byFrontiers in chemistry · 2018Article
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Authors and funding
24 authors at 3 institutions in 1 country.
Daniel L SiehlDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.) dan.siehl@pioneer.com.
Yumin TaoDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Henrik AlbertDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Yuxia DongDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Matthew HeckertDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Alfredo MadrigalDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Brishette Lincoln-CabatuDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Jian LuDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Tamara FenwickDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Ericka BermudezDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Marian SandovalDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Caroline HornDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Jerry M GreenDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Theresa HaleDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Peggy PaganoDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Jenna ClarkDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Ingrid A UdranszkyDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Nancy RizzoDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Timothy BourettDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Richard J HowardDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
David H JohnsonDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Mark VogtDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Goke AkinsolaDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Linda A CastleDuPont Pioneer, Hayward, California 94545 (D.L.S., Y.T., H.A., Y.D., M.H., A.M., B.L.-C., J.L., T.F., E.B., M.S., C.H., I.A.U., L.A.C.);DuPont Stein-Haskell Research Center, Newark, Delaware 19711 (J.M.G., T.H., P.P., J.C.);DuPont Experimental Station, Wilmington, Delaware 19803 (N.R., T.B., R.J.H.); andDuPont Pioneer, Johnston, Iowa 50131 (D.H.J., M.V., G.A.).
Pioneer Hi-Bred · USDuPont (United States) · USExperimental Station · US
Funding
No grant is acknowledged in the PubMed record.
Abstract
With an optimized expression cassette consisting of the soybean (Glycine max) native promoter modified for enhanced expression driving a chimeric gene coding for the soybean native amino-terminal 86 amino acids fused to an insensitive shuffled variant of maize (Zea mays) 4-hydroxyphenylpyruvate dioxygenase (HPPD), we achieved field tolerance in transgenic soybean plants to the HPPD-inhibiting herbicides mesotrione, isoxaflutole, and tembotrione. Directed evolution of maize HPPD was accomplished by progressively incorporating amino acids from naturally occurring diversity and novel substitutions identified by saturation mutagenesis, combined at random through shuffling. Localization of heterologously expressed HPPD mimicked that of the native enzyme, which was shown to be dually targeted to chloroplasts and the cytosol. Analysis of the native soybean HPPD gene revealed two transcription start sites, leading to transcripts encoding two HPPD polypeptides. The N-terminal region of the longer encoded peptide directs proteins to the chloroplast, while the short form remains in the cytosol. In contrast, maize HPPD was found almost exclusively in chloroplasts. Evolved HPPD enzymes showed insensitivity to five inhibitor herbicides. In 2013 field trials, transgenic soybean events made with optimized promoter and HPPD variant expression cassettes were tested with three herbicides and showed tolerance to four times the labeled rates of mesotrione and isoxaflutole and two times the labeled rates of tembotrione.
Indexed as
4-Hydroxyphenylpyruvate DioxygenaseAmino Acid SequenceCyclohexanonesGene ExpressionGlycine maxHerbicidesIsoxazolesMolecular Sequence DataPlant ProteinsPlants, Genetically ModifiedSequence Alignment4-Hydroxyphenylpyruvate DioxygenaseCyclohexanonesHerbicidesisoxaflutoleIsoxazolesPlant Proteins
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