Evidence map›Paper›PMID 25192015›Full record

ArticlePloS one2014

The expression pattern of microRNAs in granulosa cells of subordinate and dominant follicles during the early luteal phase of the bovine estrous cycle.

Dessie Salilew-Wondim, Ijaz Ahmad, Samuel Gebremedhn, Sudeep Sahadevan, M D Munir Hossain, Franca Rings, Michael Hoelker, Ernst Tholen, Christiane Neuhoff, Christian Looft and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 94 citations in OpenAlex.

  1. Genomic Analysis of Inbreeding Depression on Productive Traits in Rubia Galega Beef Cattle Breed.Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Dessie Salilew-WondimInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
Ijaz AhmadInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
Samuel GebremedhnInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
Sudeep SahadevanInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
M D Munir HossainDepartment of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Franca RingsInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
Michael HoelkerInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
Ernst TholenInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
Christiane NeuhoffInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
Christian LooftInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
Karl SchellanderInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
Dawit TesfayeInstitute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, Bonn, Germany.
University of Bonn · DEBangladesh Agricultural University · BD

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to investigate the miRNA expression patterns in granulosa cells of subordinate (SF) and dominant follicle (DF) during the early luteal phase of the bovine estrous cycle. For this, miRNA enriched total RNA isolated from granulosa cells of SF and DF obtained from heifers slaughtered at day 3 and day 7 of the estrous cycle was used for miRNAs deep sequencing. The results revealed that including 17 candidate novel miRNAs, several known miRNAs (n = 291-318) were detected in SF and DF at days 3 and 7 of the estrous cycle of which 244 miRNAs were common to all follicle groups. The let-7 families, bta-miR-10b, bta-miR-26a, bta-miR-99b and bta-miR-27b were among abundantly expressed miRNAs in both SF and DF at both days of the estrous cycle. Further analysis revealed that the expression patterns of 16 miRNAs including bta-miR-449a, bta-miR-449c and bta-miR-222 were differentially expressed between the granulosa cells of SF and DF at day 3 of the estrous cycle. However, at day 7 of the estrous cycle, 108 miRNAs including bta-miR-409a, bta-miR-383 and bta-miR-184 were differentially expressed between the two groups of granulosa cell revealing the presence of distinct miRNA expression profile changes between the two follicular stages at day 7 than day 3 of the estrous cycle. In addition, unlike the SF, marked temporal miRNA expression dynamics was observed in DF groups between day 3 and 7 of the estrous cycle. Target gene prediction and pathway analysis revealed that major signaling associated with follicular development including Wnt signaling, TGF-beta signaling, oocyte meiosis and GnRH signaling were affected by differentially expressed miRNAs. Thus, this study highlights the miRNA expression patterns of granulosa cells in subordinate and dominant follicles that could be associated with follicular recruitment, selection and dominance during the early luteal phase of the bovine estrous cycle.

Indexed as

TranscriptomeAnimalsCattleCluster AnalysisEstrous CycleFemaleGene Expression ProfilingGene Expression RegulationGranulosa CellsLuteal PhaseMicroRNAsOvarian FollicleReproducibility of ResultsRNA StabilitySignal TransductionTime FactorsMicroRNAs

Identifiers

PMID25192015
PMCPMC4156418
OpenAlexW2089923375

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.