ArticleThe ISME journal2015
Magnetic nanoparticle-mediated isolation of functional bacteria in a complex microbial community.
Article in The ISME journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 85 citations in OpenAlex.
- Influence of Ligand Functionalization on the Synthesis of Metallic-Decorated Magnetic Nanoparticles for Antibacterial Treatment.ACS applied nano materials · 2026Article
- Article
- Cellulose-functionalized magnetic nanoparticle-mediated isolation of novel cellulolytic bacteria.Applied and environmental microbiology · 2025Article
- Predictive Modeling and Experimental Validation of Magnetophoretic Delivery of Magnetic Nanocultures.ACS materials letters · 2025Article
- Promoting mechanism of nitrogen removal by FeBioprocess and biosystems engineering · 2024Article
- The effect of theApplied microbiology and biotechnology · 2024Article
- Advances and perspectives of using stable isotope probing (SIP)-based technologies in contaminant biodegradation.Water research X · 2023Article
- Hybrid magnetic core-shell TiOJournal of environmental health science & engineering · 2022Article
- Nanomaterial-assisted microfluidics for multiplex assays.Mikrochimica acta · 2022Review
- Recent advances in the application of magnetic FeEnvironmental science and pollution research international · 2021Review
- iTRAQ-Based Comparative Proteomic Analysis ofFrontiers in microbiology · 2019Article
- One-step synthesis of magnetic-TiO2-nanocomposites with high iron oxide-composing ratio for photocatalysis of rhodamine 6G.PloS one · 2019Article
- Roles of Phosphorus Sources in Microbial Community Assembly for the Removal of Organic Matters and Ammonia in Activated Sludge.Frontiers in microbiology · 2019Article
- Raman-activated cell sorting and metagenomic sequencing revealing carbon-fixing bacteria in the ocean.Environmental microbiology · 2018Article
- Diversity shift in bacterial phenol hydroxylases driven by alkyl-phenols in oil refinery wastewaters.Environmental science and pollution research international · 2017Article
- Pulse electromagnetic fields enhance extracellular electron transfer in magnetic bioelectrochemical systems.Biotechnology for biofuels · 2017Article
- Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes.The Analyst · 2016Article
- Identification of benzo[a]pyrene-metabolizing bacteria in forest soils by using DNA-based stable-isotope probing.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although uncultured microorganisms have important roles in ecosystems, their ecophysiology in situ remains elusive owing to the difficulty of obtaining live cells from their natural habitats. In this study, we employed a novel magnetic nanoparticle-mediated isolation (MMI) method to recover metabolically active cells of a group of previously uncultured phenol degraders, Burkholderiales spp., from coking plant wastewater biosludge; five other culturable phenol degraders-Rhodococcus sp., Chryseobacterium sp. and three different Pseudomonas spp.-were also isolated from the same biosludge using traditional methods. The kinetics of phenol degradation by MMI-recovered cells (MRCs) was similar to that of the original sludge. Stable isotope probing (SIP) and pyrosequencing of the 16S rRNA from the 'heavy' DNA ((13)C-DNA) fractions indicated that Burkholderiales spp. were the key phenol degraders in situ in the biosludge, consistent with the results of MRCs. Single-cell Raman micro-spectroscopy was applied to probe individual bacteria in the MRCs obtained from the SIP experiment and showed that 79% of them were fully (13)C-labelled. Biolog assays on the MRCs revealed the impact of various carbon and nitrogen substrates on the efficiency of phenol degradation in the wastewater treatment plant biosludge. Specifically, hydroxylamine, a metabolite of ammonia oxidisation, but not nitrite, nitrate or ammonia, inhibited phenol degradation in the biosludge. Our results provided a novel insight into the occasional abrupt failure events that occur in the wastewater treatment plant. This study demonstrated that MMI is a powerful tool to recover live and functional cells in situ from a complex microbial community to enable further characterisation of their physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.