ArticlePloS one2014
Uniaxial cyclic stretch promotes osteogenic differentiation and synthesis of BMP2 in the C3H10T1/2 cells with BMP2 gene variant of rs2273073 (T/G).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- The effects of mechanical stimulation on the morphology and function of spinal ligament cells.BMC musculoskeletal disorders · 2025Article
- Possible involvement of vitamin K insufficiency in the progression of cervical ossification of the posterior longitudinal ligament.Scientific reports · 2025Article
- Integrative prediction model for radiation pneumonitis incorporating genetic and clinical-pathological factors using machine learning.Clinical and translational radiation oncology · 2024Article
- Article
- The Association BetweenIndian journal of orthopaedics · 2021Article
- The Essence of Clinical Practice Guidelines for Ossification of Spinal Ligaments, 2019: 2. Pathology of OPLL.Spine surgery and related research · 2021Article
- Review
- Molecular Pathophysiology of Ossification of the Posterior Longitudinal Ligament (OPLL).Biomolecules & therapeutics · 2019Review
- A Systematic Review of Classification Systems for Cervical Ossification of the Posterior Longitudinal Ligament.Global spine journal · 2019Review
- The Pathogenesis of Ossification of the Posterior Longitudinal Ligament.Aging and disease · 2017Review
- Article
- Two novel BMP-2 variants identified in patients with thoracic ossification of the ligamentum flavum.European journal of human genetics : EJHG · 2017Article
- Lung bioengineering: physical stimuli and stem/progenitor cell biology interplay towards biofabricating a functional organ.Respiratory research · 2016Review
- Targeted next-generation sequencing reveals multiple deleterious variants in OPLL-associated genes.Scientific reports · 2016Article
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9 authors at 3 institutions in 1 country.
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Abstract
Ossification of the posterior longitudinal ligament of the cervical spine (OPLL) is characterized by the replacement of ligament tissues with ectopic bone formation, and this result is strongly affected by genetic and local factors. Two single nucleotide polymorphisms (SNPs) of rs2273073 (T/G) and rs235768 (A/T) of bone morphogenetic protein 2 (BMP2) gene which are associated with OPLL have been reported in our previous report. In this study, we confirmed the connection in 18 case samples analysis of BMP2 gene in OPLL patients; additionally, it was also shown from the OPLL patients with ligament tissues that enchondral ossification and expression of BMP2 were significantly higher compared with the non-OPLL patients by histological examination, immunohistochemistry and Western blotting analysis. To investigate the underlying mechanism, we studied the effect of SNPs in cell model. The C3H10T1/2 cells with different BMP2 gene variants were constructed and then subjected to uniaxial cyclic stretch (0.5 Hz, 10% stretch). In the presence of mechanical stress, the expression of BMP2 protein in C3H10T1/2 cells transfected by BMP2 (rs2273073 (T/G)) and BMP2 (rs2273073 (T/G), rs235768 (A/T)) were significantly higher than the corresponding static groups (P<0.05). In conclusion, these results suggested that BMP2 gene variant of rs2273073 (T/G) could not only increase cell susceptibility to bone transformation similar to pre-OPLL change, but also increase the sensibility to mechanical stress which might play an important role during the progression of OPLL.
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