ArticleNucleic acids research2014
Control of transcription elongation by GreA determines rate of gene expression in Streptococcus pneumoniae.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Inactivation of KhpB (EloR/Jag) inApplied and environmental microbiology · 2025Article
- Article
- Development of aFrontiers in microbiology · 2024Article
- Transcription-translation coupling: Recent advances and future perspectives.Molecular microbiology · 2023Article
- When push comes to shove - RNA polymerase and DNA-bound protein roadblocks.Biophysical reviews · 2023Review
- Gre factors help Salmonella adapt to oxidative stress by improving transcription elongation and fidelity of metabolic genes.PLoS biology · 2023Article
- Structural basis of RNA polymerase inhibition by viral and host factors.Nature communications · 2021Article
- Two distinct pathways of RNA polymerase backtracking determine the requirement for the Trigger Loop during RNA hydrolysis.Nucleic acids research · 2021Article
- Evolving MRSA: High-level β-lactam resistance in Staphylococcus aureus is associated with RNA Polymerase alterations and fine tuning of gene expression.PLoS pathogens · 2020Article
- Ribosome reactivates transcription by physically pushing RNA polymerase out of transcription arrest.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Probing Mechanisms of Transcription Elongation Through Cell-to-Cell Variability of RNA Polymerase.Biophysical journal · 2020Article
- High intrinsic hydrolytic activity of cyanobacterial RNA polymerase compensates for the absence of transcription proofreading factors.Nucleic acids research · 2020Article
- Involvement of Transcription Elongation Factor GreA inFrontiers in microbiology · 2020Article
- Article
- Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida.Scientific reports · 2018Article
- Transcription fidelity and its roles in the cell.Current opinion in microbiology · 2018Review
- Article
- Gre factors-mediated control of hilD transcription is essential for the invasion of epithelial cells by Salmonella enterica serovar Typhimurium.PLoS pathogens · 2017Article
- A link between transcription fidelity and pausing in vivo.Transcription · 2017Article
- Misincorporation by RNA polymerase is a major source of transcription pausing in vivo.Nucleic acids research · 2017Article
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Authors and funding
9 authors.
Funding
Abstract
Transcription by RNA polymerase may be interrupted by pauses caused by backtracking or misincorporation that can be resolved by the conserved bacterial Gre-factors. However, the consequences of such pausing in the living cell remain obscure. Here, we developed molecular biology and transcriptome sequencing tools in the human pathogen Streptococcus pneumoniae and provide evidence that transcription elongation is rate-limiting on highly expressed genes. Our results suggest that transcription elongation may be a highly regulated step of gene expression in S. pneumoniae. Regulation is accomplished via long-living elongation pauses and their resolution by elongation factor GreA. Interestingly, mathematical modeling indicates that long-living pauses cause queuing of RNA polymerases, which results in 'transcription traffic jams' on the gene and thus blocks its expression. Together, our results suggest that long-living pauses and RNA polymerase queues caused by them are a major problem on highly expressed genes and are detrimental for cell viability. The major and possibly sole function of GreA in S. pneumoniae is to prevent formation of backtracked elongation complexes.
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