ArticleCell research2014
Systematically labeling developmental stage-specific genes for the study of pancreatic β-cell differentiation from human embryonic stem cells.
Article in Cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
- SUSD2 Promotes Metastasis and Primary Tumor Growth in Pancreatic Cancer Cells via Integrin-FAK Signaling Activation.Cancer science · 2026Article
- Single-cell landscape identified SERPINB9 as a key player contributing to stemness and metastasis in non-seminomas.Cell death & disease · 2024Article
- Anti-correlated feature selection prevents false discovery of subpopulations in scRNAseq.Nature communications · 2024Article
- Inferring regulators of cell identity in the human adult pancreas.NAR genomics and bioinformatics · 2023Article
- Single-cell transcriptome analysis of NEUROG3+ cells during pancreatic endocrine differentiation with small molecules.Stem cell research & therapy · 2023Article
- Bariatric Surgery: Targeting pancreatic β cells to treat type II diabetes.Frontiers in endocrinology · 2023Review
- N-acetylcysteine regulates dental follicle stem cell osteogenesis and alveolar bone repair via ROS scavenging.Stem cell research & therapy · 2022Article
- Human pluripotent stem-cell-derived islets ameliorate diabetes in non-human primates.Nature medicine · 2022Article
- Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells.Nature communications · 2021Article
- CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells.Scientific reports · 2021Article
- Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction.Cell reports. Medicine · 2020Article
- Purification of Live Stem-Cell-Derived Islet Lineage Intermediates.Current protocols in stem cell biology · 2020Article
- Development of a Human Photoacoustic Imaging Reporter Gene Using the Clinical Dye Indocyanine Green.Radiology. Imaging cancer · 2019Article
- Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics.Molecular biotechnology · 2018Review
- Endocrine lineage biases arise in temporally distinct endocrine progenitors during pancreatic morphogenesis.Nature communications · 2018Article
- Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling.Development (Cambridge, England) · 2018Article
- Generation of carbamoyl phosphate synthetase 1 reporter cell lines for the assessment of ammonia metabolism.Journal of cellular and molecular medicine · 2017Article
- Article
- Reconstructing human pancreatic differentiation by mapping specific cell populations during development.eLife · 2017Article
- The quest to make fully functional human pancreatic beta cells from embryonic stem cells: climbing a mountain in the clouds.Diabetologia · 2016Review
Corrections and comments
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Authors and funding
21 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The applications of human pluripotent stem cell (hPSC)-derived cells in regenerative medicine has encountered a long-standing challenge: how can we efficiently obtain mature cell types from hPSCs? Attempts to address this problem are hindered by the complexity of controlling cell fate commitment and the lack of sufficient developmental knowledge for guiding hPSC differentiation. Here, we developed a systematic strategy to study hPSC differentiation by labeling sequential developmental genes to encompass the major developmental stages, using the directed differentiation of pancreatic β cells from hPSCs as a model. We therefore generated a large panel of pancreas-specific mono- and dual-reporter cell lines. With this unique platform, we visualized the kinetics of the entire differentiation process in real time for the first time by monitoring the expression dynamics of the reporter genes, identified desired cell populations at each differentiation stage and demonstrated the ability to isolate these cell populations for further characterization. We further revealed the expression profiles of isolated NGN3-eGFP(+) cells by RNA sequencing and identified sushi domain-containing 2 (SUSD2) as a novel surface protein that enriches for pancreatic endocrine progenitors and early endocrine cells both in human embryonic stem cells (hESC)-derived pancreatic cells and in the developing human pancreas. Moreover, we captured a series of cell fate transition events in real time, identified multiple cell subpopulations and unveiled their distinct gene expression profiles, among heterogeneous progenitors for the first time using our dual reporter hESC lines. The exploration of this platform and our new findings will pave the way to obtain mature β cells in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.