Evidence map›Paper›PMID 25189887›Full record

ArticleIn vivo (Athens, Greece)

Validation of an HPV16-mediated carcinogenesis mouse model.

Katherine De Azambuja, Provabati Barman, Joy Toyama, David Elashoff, Gregory W Lawson, Lisa K Williams, Kristofer Chua, Deborah Lee, Joseph J Kehoe, Andre Brodkorb and 4 more

Open access · greenAbstract readValidation Study
PubMed Full text at PMC
In one paragraph

Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 1 institution in 1 country.

Katherine De AzambujaDivision of Translational Sciences, School of Nursing, University of California at Los Angeles (UCLA), Los Angeles, CA, U.S.A.
Provabati BarmanDivision of Translational Sciences, School of Nursing, University of California at Los Angeles (UCLA), Los Angeles, CA, U.S.A.
Joy ToyamaDivision of Translational Sciences, School of Nursing, University of California at Los Angeles (UCLA), Los Angeles, CA, U.S.A.
David ElashoffDivision of General Internal Medicine and Health Services Research and David Geffen School of Medicine at UCLA, Los Angeles, CA, U.S.A.
Gregory W LawsonDepartment of Laboratory Animal Medicine, David Geffen School of Medicine at UCLA, University of California, Los Angeles, CA, U.S.A.
Lisa K WilliamsDepartment of Laboratory Animal Medicine, David Geffen School of Medicine at UCLA, University of California, Los Angeles, CA, U.S.A.
Kristofer ChuaDivision of Translational Sciences, School of Nursing, University of California at Los Angeles (UCLA), Los Angeles, CA, U.S.A.
Deborah LeeDivision of Translational Sciences, School of Nursing, University of California at Los Angeles (UCLA), Los Angeles, CA, U.S.A.
Joseph J KehoeTeagasc Food Research Centre, Moorepark, Fermoy, Ireland.
Andre BrodkorbTeagasc Food Research Centre, Moorepark, Fermoy, Ireland.
Rebecca SchwiebertCenter of Comparative Medicine, Baylor College of Medicine, Houston, TX, U.S.A.
Scott KitchenUCLA Department of Medicine, Hematology/Oncology, Los Angeles, CA, U.S.A.
Aamir BhimaniDivision of Translational Sciences, School of Nursing, University of California at Los Angeles (UCLA), Los Angeles, CA, U.S.A.
Dorothy J WileyDivision of Translational Sciences, School of Nursing, University of California at Los Angeles (UCLA), Los Angeles, CA, U.S.A. dwiley@ucla.edu.
Teagasc - The Irish Agriculture and Food Development Authority · IE

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Melissa Fitzmaurice Neligan · 1985 to 2026
$134.5M
Virology CoreP30AI028697 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MITSUYASU, RONALD T. · 1991 to 2018
$39.9M
The role of CD4 in macrophage differentiation and functionR01AI078806 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KITCHEN, SCOTT G · 2009 to 2013
$1.9M
NCI NIH HHS 5P30CA16042NCI NIH HHS P30 CA016042NIAID NIH HHS P30 AI028697NIAID NIH HHS P30 AI28697NIAID NIH HHS R01 AI078806
6 · The paper itself

Abstract

BACKGROUND/

aimHuman papillomavirus Type 16 (HPV16) infection is a necessary but alone insufficient cause of invasive cervical cancer (ICC) and likely causes other genital cancers. Individual genetic variability influences the natural history of the neoplasm. Developing a variety of animal models to investigate HPV16-mediated carcinogenesis is important to Phase 1 trials for human cancer treatments. MATERIALS AND

methodsC57BL/6 mice expressing the HPV16-E7 transgene were treated with 100 nmoles of 7,12-dimethylbenz(a)anthracene (DMBA) on dorsal-thoracolumbar skin for ≤20 weeks.

resultsTransgenic-HPV16E7 mice showed more tumors (14.11±1.49 vs. 7.2±0.73) that more quickly reached maximal size (17.53±0.53 vs. 28.75±0.67 weeks) than syngeneic controls.

conclusionDMBA topically-treated C57BL/6-HPV16E7 mice developed chronic inflammation as well as benign and malignant lesions, many of which ulcerated. Histology showed that the HPV16-E7 transgene more than doubled the effect of complete carcinogenesis against a C57BL/6 background alone, strongly influencing the number, size, and time-to-maximal tumor burden for DMBA-exposed transgenic-C57BL/6 mice.

Indexed as

Cell Transformation, ViralHuman papillomavirus 169,10-Dimethyl-1,2-benzanthraceneAnimalsBiopsyCarcinogensDisease Models, AnimalFemaleHumansMiceMice, TransgenicNeoplasmsPapillomavirus E7 ProteinsPapillomavirus InfectionsTumor Burden9,10-Dimethyl-1,2-benzanthraceneCarcinogensoncogene protein E7, Human papillomavirus type 16Papillomavirus E7 Proteins7,12-dimethylbenz(a)anthracene (DMBA)HPV16E7HPV type 16 (HPV16)micetransgenic

Identifiers

PMID25189887
PMCPMC5214601
OpenAlexW2208726328

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.