ArticleThe Journal of experimental biology2014
Dual silencing of long and short Amblyomma americanum acidic chitinase forms weakens the tick cement cone stability.
Article in The Journal of experimental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Arthropod exosomal glycine-rich protein as a potential vaccine candidate effectively reduces tick blood-feeding and pathogen transmission.The EMBO journal · 2026Article
- The salivary gland transcriptome of Varroa destructor reveals suitable targets for RNAi-based mite control.Insect molecular biology · 2025Article
- Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer.Scientific reports · 2022Article
- Borrelia burgdorferi infection modifies protein content in saliva of Ixodes scapularis nymphs.BMC genomics · 2021Article
- A salivary chitinase of Varroa destructor influences host immunity and mite's survival.PLoS pathogens · 2020Article
- Amblyomma americanum serpin 41 (AAS41) inhibits inflammation by targeting chymase and chymotrypsin.International journal of biological macromolecules · 2020Article
- Tick and Host Derived Compounds Detected in the Cement Complex Substance.Biomolecules · 2020Article
- Time-resolved proteomic profile of Amblyomma americanum tick saliva during feeding.PLoS neglected tropical diseases · 2020Article
- In silico selection of functionally important proteins from the mialome of Ornithodoros erraticus ticks and assessment of their protective efficacy as vaccine targets.Parasites & vectors · 2019Article
- Amblyomma americanum serpin 27 (AAS27) is a tick salivary anti-inflammatory protein secreted into the host during feeding.PLoS neglected tropical diseases · 2019Article
- Proteomic analysis of saliva from partially and fully engorged adult female Rhipicephalus microplus (Acari: Ixodidae).Experimental & applied acarology · 2019Article
- Genomes of trombidid mites reveal novel predicted allergens and laterally transferred genes associated with secondary metabolism.GigaScience · 2018Article
- Tick attachment cement - reviewing the mysteries of a biological skin plug system.Biological reviews of the Cambridge Philosophical Society · 2018Review
- Disruption of blood meal-responsive serpins prevents Ixodes scapularis from feeding to repletion.Ticks and tick-borne diseases · 2018Article
- Identification and characterization of proteins in the Amblyomma americanum tick cement cone.International journal for parasitology · 2018Article
- Proteomics analysis of faecal proteins in the tick Haemaphysalis flava.Parasites & vectors · 2018Article
- Tick-Host Range Adaptation: Changes in Protein Profiles in Unfed AdultFrontiers in cellular and infection microbiology · 2017Article
- Structural characterization of tick cement cones collected from in vivo and artificial membrane blood-fed Lone Star ticks (Amblyomma americanum).Ticks and tick-borne diseases · 2016Article
- Target validation of highly conserved Amblyomma americanum tick saliva serine protease inhibitor 19.Ticks and tick-borne diseases · 2016Article
- Ixodes scapularis Tick Saliva Proteins Sequentially Secreted Every 24 h during Blood Feeding.PLoS neglected tropical diseases · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
This study demonstrates that Amblyomma americanum (Aam) constitutively and ubiquitously expresses the long (L) and short (S) putative acidic chitinases (Ach) that are distinguished by a 210 base pair (bp) deletion in AamAch-S. Full-length AamAch-L and AamAch-S cDNA are 1959 and 1718 bp long, containing 1332 and 1104 bp open reading frames that code for 443 and 367 amino acid residues proteins with the former predicted to be extracellular and the latter intracellular. Both AamAch-L and AamAch-S mRNA are expressed in multiple organs as revealed by qualitative RT-PCR analysis. Furthermore, quantitative reverse transcription polymerase chain reaction analysis revealed that AamAch-L mRNA was downregulated in the mid-gut, but was unchanged in the salivary gland and in other organs in response to feeding. Of significant interest, AamAch-L and/or AamAch-S functions are probably associated with formation and/or maintenance of stability of A. americanum tick cement cone. Dual RNA interference silencing of AamAch-L and/or AamAch-S mRNA caused ticks to loosely attach onto host skin as suggested by bleeding around tick mouthparts and ticks detaching off host skin with a light touch. AamAch-L may apparently encode an inactive chitinase as indicated by Pichia pastoris-expressed recombinant AamAch-L failing to hydrolyse chitinase substrates. Unpublished related work in our laboratory, and published work by others that found AamAch-L in tick saliva, suggest that native AamAch-L is a non-specific immunoglobulin binding tick saliva protein in that rAamAch-L non-specifically bound rabbit, bovine and chicken non-immune sera. We discuss findings in this study with reference to advancing knowledge on tick feeding physiology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.