ArticleJournal of breath research2014
Volatile emanations from in vitro airway cells infected with human rhinovirus.
Article in Journal of breath research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.
- A comprehensive meta-analysis and systematic review of breath analysis in detection of COVID-19 through Volatile organic compounds.Diagnostic microbiology and infectious disease · 2024Pooled it
- Volatile Organic Compounds Induced upon Viral Infection in Cell Culture: Uniform Background Study with Use of Viruses from Different Families.Molecules (Basel, Switzerland) · 2025Article
- An engineered culture vessel and flow system to improve the in vitro analysis of volatile organic compounds.Communications engineering · 2025Article
- Bioreactor contamination monitoring using off-gassed volatile organic compounds (VOCs).Analytical and bioanalytical chemistry · 2025Article
- Zika virus modulates human fibroblasts to enhance transmission success in a controlled lab-setting.Communications biology · 2025Article
- Defining VOC signatures of airway epithelial cells with PM2.5 exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- New methods to detect bacterial or viral infections in patients with chronic obstructive pulmonary disease.Expert review of respiratory medicine · 2024Review
- High and low pathogenicity avian influenza virus discrimination and prediction based on volatile organic compounds signature by SIFT-MS: a proof-of-concept study.Scientific reports · 2024Article
- Progress and challenges of developing volatile metabolites from exhaled breath as a biomarker platform.Metabolomics : Official journal of the Metabolomic Society · 2024Review
- Volatilomes of human infection.Analytical and bioanalytical chemistry · 2024Review
- Rapid point-of-care detection of SARS-CoV-2 infection in exhaled breath using ion mobility spectrometry: a pilot study.European journal of medical research · 2023Article
- iOBPdb A Database for Experimentally Determined Functional Characterization of Insect Odorant Binding Proteins.Scientific data · 2023Article
- Microbial Volatiles as Diagnostic Biomarkers of Bacterial Lung Infection in Mechanically Ventilated Patients.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Article
- Breath Biomarkers of Influenza Infection.Open forum infectious diseases · 2022Article
- Clinical studies of detecting COVID-19 from exhaled breath with electronic nose.Scientific reports · 2022Article
- Sampling and Analysis of Low-Molecular-Weight Volatile Metabolites in Cellular Headspace and Mouse Breath.Metabolites · 2022Article
- Using trained dogs and organic semi-conducting sensors to identify asymptomatic and mild SARS-CoV-2 infections: an observational study.Journal of travel medicine · 2022Observational
- The association of wildfire air pollution with COVID-19 incidence in New South Wales, Australia.The Science of the total environment · 2022Article
- Diagnostic accuracy of non-invasive detection of SARS-CoV-2 infection by canine olfaction.PloS one · 2022Article
- Virus-induced Volatile Organic Compounds Are Detectable in Exhaled Breath during Pulmonary Infection.American journal of respiratory and critical care medicine · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Respiratory viral infections such as human rhinovirus (HRV) can lead to substantial morbidity and mortality, especially in people with underlying lung diseases such as asthma and COPD. One proposed strategy to detect viral infections non-invasively is by volatile organic compound (VOC) assessment via analysis of exhaled breath. The epithelial cells are one of the most important cell lines affected during respiratory infections as they are the first line of pathogen defense. Efforts to discover infection-specific biomarkers can be significantly aided by understanding the VOC emanations of respiratory epithelial cells. Here we test the hypothesis that VOCs obtained from the headspace of respiratory cell culture will differentiate healthy cells from those infected with HRV. Primary human tracheobronchial cells were cultured and placed in a system designed to trap headspace VOCs. HRV-infected cells were compared to uninfected control cells. In addition, cells treated with heat-killed HRV and poly(I:C), a TLR3 agonist, were compared to controls. The headspace was sampled with solid-phase microextraction fibers and VOCs were analyzed by gas chromatography/mass spectrometry. We determined differential expression of compounds such as aliphatic alcohols, branched hydrocarbons, and dimethyl sulfide by the infected cells, VOCs previously associated with oxidative stress and bacterial infection. We saw no major differences between the killed-HRV, poly(I:C), and control cell VOCs. We postulate that these compounds may serve as biomarkers of HRV infection, and that the production of VOCs is not due to TLR3 stimulation but does require active viral replication. Our novel approach may be used for the in vitro study of other important respiratory viruses, and ultimately it may aid in identifying VOC biomarkers of viral infection for point-of-care diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.