ArticleParasites & vectors2014
The relapsing fever spirochete Borrelia miyamotoi is cultivable in a modified Kelly-Pettenkofer medium, and is resistant to human complement.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
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- Establishment of a digital PCR method for detection of Borrelia burgdorferi sensu lato complex DNA in cerebrospinal fluid.Scientific reports · 2022Article
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- Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi.Scientific reports · 2021Article
- Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium.Scientific reports · 2021Article
- Review
- Borrelia miyamotoi strain LB-2001 retains plasmids and infectious phenotype throughout continuous culture passages as evaluated by multiplex PCR.Ticks and tick-borne diseases · 2021Article
- Pathogenesis of Relapsing Fever.Current issues in molecular biology · 2021Review
- Glycerophosphodiester Phosphodiesterase Identified as Non-Reliable Serological Marker forMicroorganisms · 2020Article
- Characterization of a Borrelia miyamotoi membrane antigen (BmaA) for serodiagnosis of Borrelia miyamotoi disease.Ticks and tick-borne diseases · 2020Article
- Emerging Tick-Borne Diseases.Clinical microbiology reviews · 2020Review
- Evaluation of Disease Causality of RarePathogens (Basel, Switzerland) · 2020Review
- Immunoproteomic analysis of Borrelia miyamotoi for the identification of serodiagnostic antigens.Scientific reports · 2019Article
- A newEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Review
- Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundBorrelia miyamotoi is a relapsing fever spirochete found in Ixodes ticks in North America, Europe, and Asia, and has recently been found to be invasive in humans. Cultivation of this spirochete has not yet been described, but is important for patient diagnostics and scientific purposes. Host specificity of Borrelia species is dependent on resistance to host complement (serum resistance), and since B. miyamotoi has been identified as a human pathogen we were interested whether B. miyamotoi is resistant to human complement.
methodsWe inoculated B. miyamotoi strains LB-2001 and HT31 in modified-Kelly-Pettenkofer medium with 10% fetal calf serum (MKP-F), and used standard non-laborious Borrelia culture methods to culture the spirochetes. Next, we assessed serum sensitivity by a direct killing assay and a growth inhibition assay.
resultsWe were able to passage B. miyamotoi over 10 times using a standard culture method in MKP-F medium, and found B. miyamotoi to be resistant to human complement. In contrast to B. miyamotoi, Borrelia anserina--a relapsing fever spirochete unrelated to human infection--was serum sensitive.
conclusionsUsing a variation on MKP medium we were able to culture B. miyamotoi, opening the door to in vitro research into this spirochete. In addition, we describe that B. miyamotoi is resistant to human complement, which might play an important role in pathogenesis. We have also found B. anserina to be sensitive to human complement, which might explain why it is not related to human infection. Summarizing, we describe a novel culture method for B. miyamotoi and show it is resistant to human complement.
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