ArticleJournal of the American Chemical Society2014
Crystal structure studies of RNA duplexes containing s(2)U:A and s(2)U:U base pairs.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
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- Unusual Base Pair between Two 2-Thiouridines and Its Implication for Nonenzymatic RNA Copying.Journal of the American Chemical Society · 2024Article
- Transcriptome-Wide Studies of RNA-Targeted Small Molecules Provide a Simple and Selective r(CUG)ACS central science · 2023Article
- Bio-Orthogonal Chemistry Conjugation Strategy Facilitates Investigation of N-methyladenosine and Thiouridine Guide RNA Modifications on CRISPR Activity.The CRISPR journal · 2022Article
- Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.Molecular cell · 2022Article
- High Fidelity Enzyme-Free Primer Extension with an Ethynylpyridone Thymidine Analog.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Competition between bridged dinucleotides and activated mononucleotides determines the error frequency of nonenzymatic RNA primer extension.Nucleic acids research · 2021Article
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- SARS-CoV-2 nucleocapsid and Nsp3 binding: an in silico study.Archives of microbiology · 2021Article
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- Structural insights into the mechanism of RNA recognition by the N-terminal RNA-binding domain of the SARS-CoV-2 nucleocapsid phosphoprotein.Computational and structural biotechnology journal · 2020Article
- Dicer partner protein tunes the length of miRNAs using base-mismatch in the pre-miRNA stem.Nucleic acids research · 2018Article
- Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structure and Dynamics of RNA Repeat Expansions That Cause Huntington's Disease and Myotonic Dystrophy Type 1.Biochemistry · 2017Article
- C5-substituents of uridines and 2-thiouridines present at the wobble position of tRNA determine the formation of their keto-enol or zwitterionic forms - a factor important for accuracy of reading of guanosine at the 3΄-end of the mRNA codons.Nucleic acids research · 2017Article
- Enhanced Nonenzymatic RNA Copying with 2-Aminoimidazole Activated Nucleotides.Journal of the American Chemical Society · 2017Article
- Unusual Base-Pairing Interactions in Monomer-Template Complexes.ACS central science · 2016Article
- Functional interplay between NTP leaving group and base pair recognition during RNA polymerase II nucleotide incorporation revealed by methylene substitution.Nucleic acids research · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Structural studies of modified nucleobases in RNA duplexes are critical for developing a full understanding of the stability and specificity of RNA base pairing. 2-Thio-uridine (s(2)U) is a modified nucleobase found in certain tRNAs. Thermodynamic studies have evaluated the effects of s(2)U on base pairing in RNA, where it has been shown to stabilize U:A pairs and destabilize U:G wobble pairs. Surprisingly, no high-resolution crystal structures of s(2)U-containing RNA duplexes have yet been reported. We present here two high-resolution crystal structures of heptamer RNA duplexes (5'-uagcs(2)Ucc-3' paired with 3'-aucgAgg-5' and with 3'-aucgUgg-5') containing s(2)U:A and s(2)U:U pairs, respectively. For comparison, we also present the structures of their native counterparts solved under identical conditions. We found that replacing O2 with S2 stabilizes the U:A base pair without any detectable structural perturbation. In contrast, an s(2)U:U base pair is strongly stabilized in one specific U:U pairing conformation out of four observed for the native U:U base pair. This s(2)U:U stabilization appears to be due at least in part to an unexpected sulfur-mediated hydrogen bond. This work provides additional insights into the effects of 2-thio-uridine on RNA base pairing.
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