ArticlePloS one2014
In vitro modeling of the neurovascular environment by coculturing adult human brain endothelial cells with human neural stem cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed.
- Construction and Applicability Scenarios of 3D Neurovascular Unit Models In Vitro.Biomolecules · 2026Review
- Region-Specific Adult Neural Stem Cell Niches of the Mediobasal Hypothalamus and Medulla Oblongata.Stem cell reviews and reports · 2026Review
- Reviewing vascular influences on neuronal migration, cortical development, and neurodevelopmental disorders: focus on autism, ADHD and schizophrenia.Molecular psychiatry · 2025Review
- Neural stem cells for Parkinson's disease management: Challenges, nanobased support, and prospects.World journal of stem cells · 2023Review
- Bioengineered perfused human brain microvascular networks enhance neural progenitor cell survival, neurogenesis, and maturation.Science advances · 2023Article
- Nucleolin promotes angiogenesis and endothelial metabolism along the oncofetal axis in the human brain vasculature.JCI insight · 2023Article
- Development of an In Vitro Biomimetic Peripheral Neurovascular Platform.ACS applied materials & interfaces · 2022Article
- Physical therapy exerts sub-additive and suppressive effects on intracerebral neural stem cell implantation in a rat model of stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2022Article
- Exploring the origin of the cancer stem cell niche and its role in anti-angiogenic treatment for glioblastoma.Frontiers in oncology · 2022Review
- Dynamic 3D On-Chip BBB Model Design, Development, and Applications in Neurological Diseases.Cells · 2021Review
- Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.Brain pathology (Zurich, Switzerland) · 2021Article
- Cardiovascular and Cerebrovascular Implications of Growth Restriction: Mechanisms and Potential Treatments.International journal of molecular sciences · 2021Review
- Bioengineering the neurovascular niche to study the interaction of neural stem cells and endothelial cells.APL bioengineering · 2021Review
- Sensory neurons from dorsal root ganglia regulate endothelial cell function in extracellular matrix remodelling.Cell communication and signaling : CCS · 2020Article
- Simultaneous fluorescence imaging of distinct nerve and blood vessel patterns in dual Thy1-YFP and Flt1-DsRed transgenic mice.Angiogenesis · 2020Article
- AMPK activation in pregnant human myometrial arteries from high-altitude and intrauterine growth-restricted pregnancies.American journal of physiology. Heart and circulatory physiology · 2020Article
- Characterization of gene expression changes in human neural stem cells and endothelial cells modeling a neurovascular microenvironment.Brain research bulletin · 2020Article
- Materials for blood brain barrier modelingMaterials science & engineering. R, Reports : a review journal · 2020Article
- Harnessing the anti-inflammatory properties of stem cells for transplant therapy in hemorrhagic stroke.Brain hemorrhages · 2020Article
- In Vitro Blood-Brain Barrier-Integrated Neurological Disorder Models Using a Microfluidic Device.Micromachines · 2019Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
Brain and vascular cells form a functionally integrated signalling network that is known as the neurovascular unit (NVU). The signalling (autocrine, paracrine and juxtacrine) between different elements of this unit, especially in humans, is difficult to disentangle in vivo. Developing representative in vitro models is therefore essential to better understand the cellular interactions that govern the neurovascular environment. We here describe a novel approach to assay these cellular interactions by combining a human adult cerebral microvascular endothelial cell line (hCMEC/D3) with a fetal ganglionic eminence-derived neural stem cell (hNSC) line. These cell lines provide abundant homogeneous populations of cells to produce a consistently reproducible in vitro model of endothelial morphogenesis and the ensuing NVU. Vasculature-like structures (VLS) interspersed with patches of differentiating neural cells only occurred when hNSCs were seeded onto a differentiated endothelium. These VLS emerged within 3 days of coculture and by day 6 were stabilizing. After 7 days of coculture, neuronal differentiation of hNSCs was increased 3-fold, but had no significant effect on astrocyte or oligodendrocyte differentiation. ZO1, a marker of adherens and tight junctions, was highly expressed in both undifferentiated and differentiated endothelial cells, but the adherens junction markers CD31 and VE-cadherin were significantly reduced in coculture by approximately 20%. A basement membrane, consisting of laminin, vitronectin, and collagen I and IV, separated the VLS from neural patches. This simple assay can assist in elucidating the cellular and molecular signaling involved in the formation of VLS, as well as the enhancement of neuronal differentiation through endothelial signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.