ArticleBMC genomics2014
Small non-coding RNA profiling and the role of piRNA pathway genes in the protection of chicken primordial germ cells.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- The chicken retrovirus-like geneiScience · 2026Article
- Exosomal small non-coding RNA profiling and the role of PIWI-interacting RNA pathway genes in Lumpy skin disease virus-infected bovines.Animal bioscience · 2025Article
- Primordial germ cells as a potential model for understanding (Nutri) epigenetic - metabolic interactions: a mini review.Frontiers in cell and developmental biology · 2025Review
- The first chicken oocyte nucleus whole transcriptomic profile defines the spectrum of maternal mRNA and non-coding RNA genes transcribed by the lampbrush chromosomes.Nucleic acids research · 2024Article
- Review
- Prediction of sex-determination mechanisms in avian primordial germ cells using RNA-seq analysis.Scientific reports · 2022Article
- Finer resolution analysis of transcriptional programming during the active migration of chicken primordial germ cells.Computational and structural biotechnology journal · 2022Article
- Time-Course Transcriptional and Chromatin Accessibility Profiling Reveals Genes Associated With Asymmetrical Gonadal Development in Chicken Embryos.Frontiers in cell and developmental biology · 2022Article
- Zygotic genome activation in the chicken: a comparative review.Cellular and molecular life sciences : CMLS · 2020Review
- In vitro culture and characterization of duck primordial germ cells.Poultry science · 2019Article
- Rapid evolution and conserved function of the piRNA pathway.Open biology · 2019Review
- Genome-Wide Identification and Transcriptomic Analysis of MicroRNAs Across Various Amphioxus Organs Using Deep Sequencing.Frontiers in genetics · 2019Article
- Stage-dependent piRNAs in chicken implicated roles in modulating male germ cell development.BMC genomics · 2018Article
- piRNA cluster database: a web resource for piRNA producing loci.Nucleic acids research · 2016Article
- Germline Modification and Engineering in Avian Species.Molecules and cells · 2015Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGenes, RNAs, and proteins play important roles during germline development. However, the functions of non-coding RNAs (ncRNAs) on germline development remain unclear in avian species. Recent high-throughput techniques have identified several classes of ncRNAs, including micro RNAs (miRNAs), small-interfering RNAs (siRNAs), and PIWI-interacting RNAs (piRNAs). These ncRNAs are functionally important in the genome, however, the identification and annotation of ncRNAs in a genome is challenging. The aim of this study was to identify different types of small ncRNAs particularly piRNAs, and the role of piRNA pathway genes in the protection of chicken primordial germ cells (PGCs).
resultsAt first, we performed next-generation sequencing to identify ncRNAs in chicken PGCs, and we performed ab initio predictive analysis to identify putative piRNAs in PGCs. Then, we examined the expression of three repetitive sequence-linked piRNAs and 14 genic-transcript-linked piRNAs along with their linked genes using real-time PCR. All piRNAs and their linked genes were highly expressed in PGCs. Subsequently, we knocked down two known piRNA pathway genes of chicken, PIWI-like protein 1 (CIWI) and 2 (CILI), in PGCs using siRNAs. After knockdown of CIWI and CILI, we examined their effects on the expression of six putative piRNA-linked genes and DNA double-strand breakage in PGCs. The knockdown of CIWI and CILI upregulated chicken repetitive 1 (CR1) element and RAP2B, a member of RAS oncogene family, and increased DNA double-strand breakage in PGCs.
conclusionsOur results increase the understanding of PGC-expressed piRNAs and the role of piRNA pathway genes in the protection of germ cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.