ArticleStructure (London, England : 1993)2014
Dramatic improvement of crystals of large RNAs by cation replacement and dehydration.
Article in Structure (London, England : 1993), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Advances in solid-state NMR methods for studying RNA structures and dynamics.Magnetic resonance letters · 2025Review
- A general strategy for engineering GU base pairs to facilitate RNA crystallization.Nucleic acids research · 2025Article
- Riboswitch Mechanisms for Regulation of P1 Helix Stability.International journal of molecular sciences · 2024Review
- RNAvigate: efficient exploration of RNA chemical probing datasets.Nucleic acids research · 2024Article
- Single-Molecule Correlated Chemical Probing: A Revolution in RNA Structure Analysis.Accounts of chemical research · 2023Article
- Article
- Cotranscriptional Assembly and Native Purification of Large RNA-RNA Complexes for Structural Analyses.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Dynamics of base pairs with low stability in RNA by solid-state nuclear magnetic resonance exchange spectroscopy.iScience · 2022Article
- Potential effects of metal ion induced two-state allostery on the regulatory mechanism of add adenine riboswitch.Communications biology · 2022Article
- Discovery of a large-scale, cell-state-responsive allosteric switch in the 7SK RNA using DANCE-MaP.Molecular cell · 2022Article
- Rational engineering enables co-crystallization and structural determination of the HIV-1 matrix-tRNA complex.STAR protocols · 2022Article
- Predicting RNA Secondary Structure Using In Vitro and In Vivo Data.Methods in molecular biology (Clifton, N.J.) · 2022Article
- CoCoNet-boosting RNA contact prediction by convolutional neural networks.Nucleic acids research · 2021Article
- Structures of artificially designed discrete RNA nanoarchitectures at near-atomic resolution.Science advances · 2021Article
- Engineering Crystal Packing in RNA Structures I: Past and Future Strategies for Engineering RNA Packing in Crystals.Crystals · 2021Article
- Thermostability, Tunability, and Tenacity of RNA as Rubbery Anionic Polymeric Materials in Nanotechnology and Nanomedicine-Specific Cancer Targeting with Undetectable Toxicity.Chemical reviews · 2021Review
- Article
- Optimized Magnesium Force Field Parameters for Biomolecular Simulations with Accurate Solvation, Ion-Binding, and Water-Exchange Properties.Journal of chemical theory and computation · 2021Article
- Article
- Improving RNA Crystal Diffraction Quality by Postcrystallization Treatment.Methods in molecular biology (Clifton, N.J.) · 2021Article
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2 authors.
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Abstract
Compared to globular proteins, RNAs with complex 3D folds are characterized by poorly differentiated molecular surfaces dominated by backbone phosphates, sparse tertiary contacts stabilizing global architecture, and conformational flexibility. The resulting generally poor order of crystals of large RNAs and their complexes frequently hampers crystallographic structure determination. We describe and rationalize a postcrystallization treatment strategy that exploits the importance of solvation and counterions for RNA folding. Replacement of Li(+) and Mg(2+) needed for growth of crystals of a tRNA-riboswitch-protein complex with Sr(2+), coupled with dehydration, dramatically improved the resolution limit (8.5-3.2 Å) and data quality, enabling structure determination. The soft Sr(2+) ion forms numerous stabilizing intermolecular contacts. Comparison of pre- and posttreatment structures reveals how RNA assemblies redistribute as quasi-rigid bodies to yield improved crystal packing. Cation exchange complements previously reported postcrystallization dehydration of protein crystals and represents a potentially general strategy for improving crystals of large RNAs.
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