ArticleBiophysical journal2014
Single lipid extraction: the anchoring strength of cholesterol in liquid-ordered and liquid-disordered phases.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Controlling membrane tension by dynamic DNA nanorings.bioRxiv : the preprint server for biology · 2026Article
- Design of a Trigger-Responsive Photothermal Vesicle-Based Cargo Delivery Platform.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- DNA origami nano-mechanics.Chemical Society reviews · 2021Review
- Measurement of Single-Molecule Forces in Cholesterol and Cyclodextrin Host-Guest Complexes.The journal of physical chemistry. B · 2021Article
- Towards a Quantitative Understanding of Protein-Lipid Bilayer Interactions at the Single Molecule Level: Opportunities and Challenges.The Journal of membrane biology · 2021Review
- Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics.APL bioengineering · 2020Review
- Calcium-triggered fusion of lipid membranes is enabled by amphiphilic nanoparticles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Quantifying tensile forces at cell-cell junctions with a DNA-based fluorescent probe.Chemical science · 2020Article
- Modelling of interactions between Aβ(25-35) peptide and phospholipid bilayers: effects of cholesterol and lipid saturation.RSC advances · 2020Article
- Investigating single molecule adhesion by atomic force spectroscopy.Journal of visualized experiments : JoVE · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cholesterol is important for the formation of microdomains in supported lipid bilayers and is enriched in the liquid-ordered phase. To understand the interactions leading to this enrichment, we developed an AFM-based single-lipid-extraction (SLX) approach that enables us to determine the anchoring strength of cholesterol in the two phases of a phase-separated lipid membrane. As expected, the forces necessary for extracting a single cholesterol molecule from liquid-ordered phases are significantly higher than for extracting it from the liquid-disordered phases. Interestingly, application of the Bell model shows two energy barriers that correlate with the head and full length of the cholesterol molecule. The resulting lifetimes for complete extraction are 90 s and 11 s in the liquid-ordered and liquid-disordered phases, respectively. Molecular dynamics simulations of the very same experiment show similar force profiles and indicate that the stabilization of cholesterol in the liquid-ordered phase is mainly due to nonpolar contacts.
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