ArticleBiophysical journal2014
Analytic 3D imaging of mammalian nucleus at nanoscale using coherent x-rays and optical fluorescence microscopy.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Combined optical fluorescence microscopy and X-ray tomography reveals substructures in cell nuclei in 3D.Biomedical optics express · 2022Article
- Stochastic chromatin packing of 3D mitotic chromosomes revealed by coherent X-rays.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Methods and application of coherent X-ray diffraction imaging of noncrystalline particles.Biophysical reviews · 2020Review
- Spectral comparisons of mammalian cells and intact organelles by solid-state NMR.Journal of structural biology · 2019Article
- Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge.Scientific reports · 2017Article
- Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging.Scientific reports · 2017Article
- Tomography of a Cryo-immobilized Yeast Cell Using Ptychographic Coherent X-Ray Diffractive Imaging.Biophysical journal · 2015Article
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Authors and funding
7 authors.
Funding
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Abstract
Despite the notable progress that has been made with nano-bio imaging probes, quantitative nanoscale imaging of multistructured specimens such as mammalian cells remains challenging due to their inherent structural complexity. Here, we successfully performed three-dimensional (3D) imaging of mammalian nuclei by combining coherent x-ray diffraction microscopy, explicitly visualizing nuclear substructures at several tens of nanometer resolution, and optical fluorescence microscopy, cross confirming the substructures with immunostaining. This demonstrates the successful application of coherent x-rays to obtain the 3D ultrastructure of mammalian nuclei and establishes a solid route to nanoscale imaging of complex specimens.
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