ArticleBiophysical journal2014
Explicit spatiotemporal simulation of receptor-G protein coupling in rod cell disk membranes.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 39 citations in OpenAlex.
- Supramolecular organization of rhodopsin in rod photoreceptor cell membranes.Pflugers Archiv : European journal of physiology · 2021Review
- A hybrid stochastic/deterministic model of single photon response and light adaptation in mouse rods.Computational and structural biotechnology journal · 2021Article
- Rhodopsin Oligomerization and Aggregation.The Journal of membrane biology · 2019Review
- ReaDDy 2: Fast and flexible software framework for interacting-particle reaction dynamics.PLoS computational biology · 2019Article
- It takes two transducins to activate the cGMP-phosphodiesterase 6 in retinal rods.Open biology · 2018Article
- Excluded volume effects in on- and off-lattice reaction-diffusion models.IET systems biology · 2017Article
- Reaction-diffusion basis of retroviral infectivity.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
- Molecular basis for photoreceptor outer segment architecture.Progress in retinal and eye research · 2016Review
- Quaternary structures of opsin in live cells revealed by FRET spectrometry.The Biochemical journal · 2016Article
- Membrane omega-3 fatty acids modulate the oligomerisation kinetics of adenosine A2A and dopamine D2 receptors.Scientific reports · 2016Article
- Dynamical Organization of Syntaxin-1A at the Presynaptic Active Zone.PLoS computational biology · 2015Article
- Response to comment "Transient complexes between dark rhodopsin and transducin: circumstantial evidence or physiological necessity?" by D. Dell'Orco and K.-W. Koch.Biophysical journal · 2015Article
- Transient complexes between dark rhodopsin and transducin: circumstantial evidence or physiological necessity?Biophysical journal · 2015Article
- ReaDDyMM: Fast interacting particle reaction-diffusion simulations using graphical processing units.Biophysical journal · 2015Article
- Nanodomain organization of rhodopsin in native human and murine rod outer segment disc membranes.Biochimica et biophysica acta · 2015Article
- Protein and Signaling Networks in Vertebrate Photoreceptor Cells.Frontiers in molecular neuroscience · 2015Review
- Simulation tools for particle-based reaction-diffusion dynamics in continuous space.BMC biophysics · 2014Review
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4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dim-light vision is mediated by retinal rod cells. Rhodopsin (R), a G-protein-coupled receptor, switches to its active form (R(∗)) in response to absorbing a single photon and activates multiple copies of the G-protein transducin (G) that trigger further downstream reactions of the phototransduction cascade. The classical assumption is that R and G are uniformly distributed and freely diffusing on disk membranes. Recent experimental findings have challenged this view by showing specific R architectures, including RG precomplexes, nonuniform R density, specific R arrangements, and immobile fractions of R. Here, we derive a physical model that describes the first steps of the photoactivation cascade in spatiotemporal detail and single-molecule resolution. The model was implemented in the ReaDDy software for particle-based reaction-diffusion simulations. Detailed kinetic in vitro experiments are used to parametrize the reaction rates and diffusion constants of R and G. Particle diffusion and G activation are then studied under different conditions of R-R interaction. It is found that the classical free-diffusion model is consistent with the available kinetic data. The existence of precomplexes between inactive R and G is only consistent with the data if these precomplexes are weak, with much larger dissociation rates than suggested elsewhere. Microarchitectures of R, such as dimer racks, would effectively immobilize R but have little impact on the diffusivity of G and on the overall amplification of the cascade at the level of the G protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.