ArticlePlant physiology2014
In vivo ³¹P-nuclear magnetic resonance studies of glyphosate uptake, vacuolar sequestration, and tonoplast pump activity in glyphosate-resistant horseweed.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 64 citations in OpenAlex.
- Thirty years of glyphosate-resistant crops and weeds: Current situation and future prospects.Pest management science · 2026Review
- Functional synergy and genomic linkage of glyphosate resistance traits in Canada fleabane.Pest management science · 2026Article
- Article
- An ABC transporter of the ABCC subfamily localized at the plasma membrane confers glyphosate resistance.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- First Case of Glyphosate Resistance inFrontiers in plant science · 2021Article
- Article
- Evolution of Glyphosate-Resistant Weeds.Reviews of environmental contamination and toxicology · 2021Review
- The First Case of Glyphosate Resistance in Johnsongrass (Plants (Basel, Switzerland) · 2020Article
- Non-target-Site Resistance inFrontiers in plant science · 2020Review
- Non-Target-Site Resistance to Herbicides: Recent Developments.Plants (Basel, Switzerland) · 2019Review
- The Triple Amino Acid Substitution TAP-IVS in theInternational journal of molecular sciences · 2019Article
- Comparative proteomic analysis of horseweed (Conyza canadensis) biotypes identifies candidate proteins for glyphosate resistance.Scientific reports · 2017Article
- Differential Resistance Mechanisms to Glyphosate Result in Fitness Cost forFrontiers in plant science · 2017Article
- Article
- Protein kinase GCN2 mediates responses to glyphosate in Arabidopsis.BMC plant biology · 2015Article
- Focus on weed control.Plant physiology · 2014Article
- De novo genome assembly of the economically important weed horseweed using integrated data from multiple sequencing platforms.Plant physiology · 2014Article
Corrections and comments
- Erratum issuedCorrections.2015
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Horseweed (Conyza canadensis) is considered a significant glyphosate-resistant (GR) weed in agriculture, spreading to 21 states in the United States and now found globally on five continents. This laboratory previously reported rapid vacuolar sequestration of glyphosate as the mechanism of resistance in GR horseweed. The observation of vacuole sequestration is consistent with the existence of a tonoplast-bound transporter. (31)P-Nuclear magnetic resonance experiments performed in vivo with GR horseweed leaf tissue show that glyphosate entry into the plant cell (cytosolic compartment) is (1) first order in extracellular glyphosate concentration, independent of pH and dependent upon ATP; (2) competitively inhibited by alternative substrates (aminomethyl phosphonate [AMPA] and N-methyl glyphosate [NMG]), which themselves enter the plant cell; and (3) blocked by vanadate, a known inhibitor/blocker of ATP-dependent transporters. Vacuole sequestration of glyphosate is (1) first order in cytosolic glyphosate concentration and dependent upon ATP; (2) competitively inhibited by alternative substrates (AMPA and NMG), which themselves enter the plant vacuole; and (3) saturable. (31)P-Nuclear magnetic resonance findings with GR horseweed are consistent with the active transport of glyphosate and alternative substrates (AMPA and NMG) across the plasma membrane and tonoplast in a manner characteristic of ATP-binding cassette transporters, similar to those that have been identified in mammalian cells.
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