ArticleJournal of experimental botany2015
Chloroplast-encoded serotonin N-acetyltransferase in the red alga Pyropia yezoensis: gene transition to the nucleus from chloroplasts.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- Melatonin-Producing Microorganisms: A Rising Research Interest in Their Melatonin Biosynthesis and Effects on Crops.Journal of pineal research · 2025Review
- Role of Neurotransmitters (Biomediators) in Plant Responses to Stress.Plants (Basel, Switzerland) · 2024Review
- Article
- Melatonin Involved in Protective Effects against Cadmium Stress inInternational journal of molecular sciences · 2023Article
- Functional Characterization of ArylalkylamineAntioxidants (Basel, Switzerland) · 2022Article
- Melatonin biosynthesis pathways in nature and its production in engineered microorganisms.Synthetic and systems biotechnology · 2022Review
- Genome-wide identification and characteristic analysis of the downstream melatonin metabolism gene GhM2H in Gossypium hirsutum L.Biological research · 2021Article
- Melatonin and Phytomelatonin: Chemistry, Biosynthesis, Metabolism, Distribution and Bioactivity in Plants and Animals-An Overview.International journal of molecular sciences · 2021Review
- Melatonin Regulates Chloroplast Protein Quality Control via a Mitogen-Activated Protein Kinase Signaling Pathway.Antioxidants (Basel, Switzerland) · 2021Article
- Functional Characterization of Serotonin N-Acetyltransferase Genes (Frontiers in plant science · 2021Article
- Genome-Wide Identification and Expression Profile of the SNAT Gene Family in Tobacco (Frontiers in genetics · 2020Article
- Melatonin Synthesis and Function: Evolutionary History in Animals and Plants.Frontiers in endocrinology · 2019Review
- Article
- Melatonin transport into mitochondria.Cellular and molecular life sciences : CMLS · 2017Review
- Pyropia yezoensis peptide PYP1‑5 protects against dexamethasone‑induced muscle atrophy through the downregulation of atrogin1/MAFbx and MuRF1 in mouse C2C12 myotubes.Molecular medicine reports · 2017Article
- Melatonin: A Mitochondrial Targeting Molecule Involving Mitochondrial Protection and Dynamics.International journal of molecular sciences · 2016Review
- Immunohistochemical and ultrastructural properties of the larval ciliary band-associated strand in the sea urchin Hemicentrotus pulcherrimus.Frontiers in zoology · 2016Article
- Endophytic BacteriumFrontiers in plant science · 2016Article
- Melatonin in Plants and Plant Culture Systems: Variability, Stability and Efficient Quantification.Frontiers in plant science · 2016Article
- Melatonin-Producing Endophytic Bacteria from Grapevine Roots Promote the Abiotic Stress-Induced Production of Endogenous Melatonin in Their Hosts.Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Melatonin biosynthesis involves the N-acetylation of arylalkylamines such as serotonin, which is catalysed by serotonin N-acetyltransferase (SNAT), the penultimate enzyme of melatonin biosynthesis in both animals and plants. Here, we report the functional characterization of a putative N-acetyltransferase gene in the chloroplast genome of the alga laver (Pyropia yezoensis, formerly known as Porphyra yezoensis) with homology to the rice SNAT gene. To confirm that the putative Pyropia yezoensis SNAT (PySNAT) gene encodes an SNAT, we cloned the full-length chloroplastidic PySNAT gene by PCR and purified the recombinant PySNAT protein from Escherichia coli. PySNAT was 174 aa and had 50% amino acid identity with cyanobacteria SNAT. Purified recombinant PySNAT showed a peak activity at 55 °C with a K m of 467 µM and V max of 28 nmol min-1 mg(-1) of protein. Unlike other plant SNATs, PySNAT localized to the cytoplasm due to a lack of N-terminal chloroplast transit peptides. Melatonin was present at 0.16ng g(-1) of fresh mass but increased during heat stress. Phylogenetic analysis of the sequence suggested that PySNAT has evolved from the cyanobacteria SNAT gene via endosymbiotic gene transfer. Additionally, the chloroplast transit peptides of plant SNATs were acquired 1500 million years ago, concurrent with the appearance of green algae.
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