ArticleAsian Pacific journal of tropical biomedicine2014
PCR-based identification of methicillin-resistant Staphylococcus aureus strains and their antibiotic resistance profiles.
Article in Asian Pacific journal of tropical biomedicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Epidemiology of clinically isolated methicillin-resistant Staphylococcus aureus (MRSA) and its susceptibility to linezolid and vancomycin in Egypt: a systematic review with meta-analysis.BMC infectious diseases · 2023Pooled it
- Hemolytic activity and antibiotic resistance profiles ofMicrobiology spectrum · 2026Article
- Phenotypic and Genotypic Analyses of Antimicrobial Resistance Patterns ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2026Article
- High-level multidrug resistance and an unexpected mecA gene detection in Vibrio spp. from Litopenaeus vannamei aquaculture in Vietnam.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Antimicrobial Activity of Greek Native Essential Oils AgainstAntibiotics (Basel, Switzerland) · 2025Article
- Whole Genome Sequence Analysis of Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Article
- Antibacterial activity of selenium nanoparticles/copper oxide (SeNPs/CuO) nanocomposite against some multi-drug resistant clinical pathogens.BMC microbiology · 2025Article
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- Synthesis, characterization, quantum chemical modelling, molecular docking, in silico and in vitro assessment of 3-(2-bromo-5-fluorophenyl))-1-(thiophen-2-yl)prop-2-en-1-one.Scientific reports · 2024Article
- Assessment of the presence of multidrug-resistantHeliyon · 2024Article
- Performance evaluation of cefoxitin screen test on two different automated antimicrobial susceptibility test systems: a comparative study.Microbiology spectrum · 2024Article
- Development of triplex assay for simultaneous detection ofJournal of food science and technology · 2024Article
- Screening for Antibiotic Resistance Genes in Bacteria and the Presence of Heavy Metals in the Upstream and Downstream Areas of the Wadi Hanifah Valley in Riyadh, Saudi Arabia.Antibiotics (Basel, Switzerland) · 2024Article
- Antimicrobial resistance markers distribution in Staphylococcus aureus from Nsukka, Nigeria.BMC infectious diseases · 2024Article
- Exploration ofFrontiers in cellular and infection microbiology · 2024Article
- Molecular Epidemiology of Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- Antiseptics and mupirocin resistance in clinical, environmental, and colonizing coagulase negative Staphylococcus isolates.Antimicrobial resistance and infection control · 2023Article
- Antibiotic Resistance Mediated byAntibiotics (Basel, Switzerland) · 2023Article
- Insight into the Clonal Lineage and Antimicrobial Resistance ofAntibiotics (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo evaluated the PCR for mecA gene compared with the conventional oxacillin disk diffusion method for methicillin-resistant Staphylococcus aureus (S. aureus) identification.
methodsA total of 292 S. aureus strains were isolated from various clinical specimens obtained from hospitalized patients. Susceptibility test to several antimicrobial agents was performed by disk diffusion agar according to Clinical and Laboratory Standards Institute guidelines. The PCR amplification of the mecA gene was carried out in all the clinical isolates.
resultsAmong antibiotics used in our study, penicillin showed the least anti-staphylococcal activity and vancomycin was the most effective. The rate of methicillin-resistant S. aureus prevalence determined by oxacillin disk diffusion method was 47.6%; whereas, 45.1% of S. aureus isolates were mecA- positive in the PCR assay.
conclusionsThis study is suggestive that the PCR for detection of mecA gene is a fast, accurate and valuable diagnostic tool, particularly in hospitals in areas where methicillin-resistant S. aureus is endemic.
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