ArticleThe Journal of biological chemistry2014
Metabolite profiling and integrative modeling reveal metabolic constraints for carbon partitioning under nitrogen starvation in the green algae Haematococcus pluvialis.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 143 citations in OpenAlex.
- Synergistic Application of Cytidine Monophosphate and Sodium Chloride for Enhanced Co-Production of Astaxanthin and Fatty Acids inMarine drugs · 2026Article
- Chlamydomonas reinhardtii, Volvox carteri and related green algae accumulate ketocarotenoids not in vegetative cells but in zygospores.The Plant journal : for cell and molecular biology · 2025Article
- Charting the state of GEMs in microalgae: progress, challenges, and innovations.Frontiers in plant science · 2025Review
- Article
- Integrated Transcriptomics and Metabolomics Analysis Reveal the Regulatory Mechanisms Underlying Sodium Butyrate-Induced Carotenoid Biosynthesis inJournal of fungi (Basel, Switzerland) · 2024Article
- Influence of light absorption rate on the astaxanthin production by the microalga Haematococcus pluvialis during nitrogen starvation.Bioresources and bioprocessing · 2023Article
- Astaxanthin: Past, Present, and Future.Marine drugs · 2023Review
- Identification and Characterization of a New MicroalgaBiology · 2022Article
- Balancing Damage via Non-Photochemical Quenching, Phenolic Compounds and Photorespiration inInternational journal of molecular sciences · 2022Article
- Integration of the metabolome and transcriptome reveals the mechanism of resistance to low nitrogen supply in wild bermudagrass (Cynodon dactylon (L.) Pers.) roots.BMC plant biology · 2021Article
- Microalgae as Sustainable Biofactories to Produce High-Value Lipids: Biodiversity, Exploitation, and Biotechnological Applications.Marine drugs · 2021Review
- E/Z isomerization of astaxanthin and its monoesters in vitro under the exposure to light or heat and in overilluminated Haematococcus pluvialis cells.Bioresources and bioprocessing · 2021Article
- Characterization of type-2 diacylglycerol acyltransferases in Haematococcus lacustris reveals their functions and engineering potential in triacylglycerol biosynthesis.BMC plant biology · 2021Article
- Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling.RSC advances · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation inFrontiers in bioengineering and biotechnology · 2020Article
- Increased urea availability promotes adjustments in C/N metabolism and lipid content without impacting growth in Chlamydomonas reinhardtii.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- Comparative transcriptome analysis of a long-time span two-step culture process reveals a potential mechanism for astaxanthin and biomass hyper-accumulation inBiotechnology for biofuels · 2019Article
- A hypothesis about the origin of carotenoid lipid droplets in the green algae Dunaliella and Haematococcus.Planta · 2019Review
- Proteomic Data Integration Highlights Central Actors Involved in Einkorn (Frontiers in plant science · 2019Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The green alga Hematococcus pluvialis accumulates large amounts of the antioxidant astaxanthin under inductive stress conditions, such as nitrogen starvation. The response to nitrogen starvation and high light leads to the accumulation of carbohydrates and fatty acids as well as increased activity of the tricarboxylic acid cycle. Although the behavior of individual pathways has been well investigated, little is known about the systemic effects of the stress response mechanism. Here we present time-resolved metabolite, enzyme activity, and physiological data that capture the metabolic response of H. pluvialis under nitrogen starvation and high light. The data were integrated into a putative genome-scale model of the green alga to in silico test hypotheses of underlying carbon partitioning. The model-based hypothesis testing reinforces the involvement of starch degradation to support fatty acid synthesis in the later stages of the stress response. In addition, our findings support a possible mechanism for the involvement of the increased activity of the tricarboxylic acid cycle in carbon repartitioning. Finally, the in vitro experiments and the in silico modeling presented here emphasize the predictive power of large scale integrative approaches to pinpoint metabolic adjustment to changing environments.
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