ArticleAsian Pacific journal of tropical biomedicine2014
Rapid detection of coliforms in drinking water of Arak city using multiplex PCR method in comparison with the standard method of culture (Most Probably Number).
Article in Asian Pacific journal of tropical biomedicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 24 citations in OpenAlex.
- The presence of SARS-CoV-2 RNA in different freshwater environments in urban settings determined by RT-qPCR: Implications for water safety.The Science of the total environment · 2021Article
- Microbiological Assessment of Tap Water Following the 2016 Louisiana Flooding.International journal of environmental research and public health · 2020Article
- Antimicrobial, Optical and Mechanical Properties of Chitosan-Starch Films with Natural Extracts.International journal of molecular sciences · 2017Article
- Evaluation of Polymerase Chain Reaction for Detecting Coliform Bacteria in Drinking Water Sources.Advanced biomedical research · 2017Article
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Authors and funding
12 authors at 5 institutions in 1 country.
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Abstract
objectiveTo analyse molecular detection of coliforms and shorten the time of PCR.
methodsRapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in comparison with most probably number (MPN) method for 16 artificial and 101 field samples. The molecular method was also conducted on isolated coliforms from positive MPN samples; standard sample for verification of microbial method certificated reference material; isolated strains from certificated reference material and standard bacteria. The PCR and electrophoresis parameters were changed for reducing the operation time.
resultsResults of PCR for lacZ and uidA genes were similar in all of standard, operational and artificial samples and showed the 876 bp and 147 bp bands of lacZ and uidA genes by multiplex PCR. PCR results were confirmed by MPN culture method by sensitivity 86% (95% CI: 0.71-0.93). Also the total execution time, with a successful change of factors, was reduced to less than two and a half hour.
conclusionsMultiplex PCR method with shortened operation time was used for the simultaneous detection of total coliforms and Escherichia coli in distribution system of Arak city. It's recommended to be used at least as an initial screening test, and then the positive samples could be randomly tested by MPN.
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