ArticleAntimicrobial agents and chemotherapy2014
Disruption of the transcriptional regulator Cas5 results in enhanced killing of Candida albicans by Fluconazole.
Article in Antimicrobial agents and chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
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- [Electroencephalographic microstates in vestibular schwannoma patients with tinnitus].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2023Article
- The proteasome regulator Rpn4 controls antifungal drug tolerance by coupling protein homeostasis with metabolic responses to drug stress.PLoS pathogens · 2023Article
- Study on tinnitus-related electroencephalogram microstates in patients with vestibular schwannomas.Frontiers in neuroscience · 2023Article
- What 'Omics can tell us about antifungal adaptation.FEMS yeast research · 2022Review
- Etest ECVs/ECOFFs for Detection of Resistance in Prevalent and Three NonprevalentAntimicrobial agents and chemotherapy · 2021Article
- The transcription factor Cas5 suppresses hyphal morphogenesis during yeast-form growth in Candida albicans.Journal of microbiology (Seoul, Korea) · 2021Article
- Fungal Cell Wall Proteins and Signaling Pathways Form a Cytoprotective Network to Combat Stresses.Journal of fungi (Basel, Switzerland) · 2021Review
- Efg1 and Cas5 Orchestrate Cell Wall Damage Response to Caspofungin in Candida albicans.Antimicrobial agents and chemotherapy · 2021Article
- Transcriptional regulation of the caspofungin-induced cell wall damage response in Candida albicans.Current genetics · 2020Review
- An expanded cell wall damage signaling network is comprised of the transcription factors Rlm1 and Sko1 in Candida albicans.PLoS genetics · 2020Article
- Identification and Characterization of Mediators of Fluconazole Tolerance inFrontiers in microbiology · 2020Article
- Transcriptional Circuits Regulating Developmental Processes inFrontiers in cellular and infection microbiology · 2020Review
- Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review.Journal of fungi (Basel, Switzerland) · 2019Review
- Article
- Linking Cellular Morphogenesis with Antifungal Treatment and Susceptibility inJournal of fungi (Basel, Switzerland) · 2019Review
- Host-Pathogen Interactions Mediated by MDR Transporters in Fungi: As Pleiotropic as it Gets!Genes · 2018Review
- Antifungal tolerance is a subpopulation effect distinct from resistance and is associated with persistent candidemia.Nature communications · 2018Article
- Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Azole antifungal agents such as fluconazole exhibit fungistatic activity against Candida albicans. Strategies to enhance azole antifungal activity would be therapeutically appealing. In an effort to identify transcriptional pathways that influence the killing activity of fluconazole, we sought to identify transcription factors (TFs) involved in this process. From a collection of C. albicans strains disrupted for genes encoding TFs (O. R. Homann, J. Dea, S. M. Noble, and A. D. Johnson, PLoS Genet. 5:e1000783, 2009, http://dx.doi.org/10.1371/journal.pgen.1000783), four strains exhibited marked reductions in minimum fungicidal concentration (MFCs) in both RPMI and yeast extract-peptone-dextrose (YPD) media. One of these genes, UPC2, was previously characterized with regard to its role in azole susceptibility. Of mutants representing the three remaining TF genes of interest, one (CAS5) was unable to recover from fluconazole exposure at concentrations as low as 2 μg/ml after 72 h in YPD medium. This mutant also showed reduced susceptibility and a clear zone of inhibition by Etest, was unable to grow on solid medium containing 10 μg/ml fluconazole, and exhibited increased susceptibility by time-kill analysis. CAS5 disruption in highly azole-resistant clinical isolates exhibiting multiple resistance mechanisms did not alter susceptibility. However, CAS5 disruption in strains with specific resistance mutations resulted in moderate reductions in MICs and MFCs. Genome-wide transcriptional analysis was performed in the presence of fluconazole and was consistent with the suggested role of CAS5 in cell wall organization while also suggesting a role in iron transport and homeostasis. These findings suggest that Cas5 regulates a transcriptional network that influences the response of C. albicans to fluconazole. Further delineation of this transcriptional network may identify targets for potential cotherapeutic strategies to enhance the activity of the azole class of antifungals.
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