ArticleJournal of bacteriology2014
Cell separation in Vibrio cholerae is mediated by a single amidase whose action is modulated by two nonredundant activators.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 75 citations in OpenAlex.
- Outer membrane barrier impairment byMicrobiology (Reading, England) · 2024Article
- Lytic transglycosylase repertoire diversity enables intrinsic antibiotic resistance and daughter cell separation inAntimicrobial agents and chemotherapy · 2024Article
- EnvC Homolog Encoded byMicroorganisms · 2024Article
- Structure predictions and functional insights into Amidase_3 domain containing N-acetylmuramyl-L-alanine amidases from Deinococcus indicus DR1.BMC microbiology · 2024Article
- DipM controls multiple autolysins and mediates a regulatory feedback loop promoting cell constriction in Caulobacter crescentus.Nature communications · 2023Article
- Regulation of peptidoglycan hydrolases: localization, abundance, and activity.Current opinion in microbiology · 2023Review
- Disrupting Central Carbon Metabolism Increases β-Lactam Antibiotic Susceptibility in Vibrio cholerae.Journal of bacteriology · 2023Article
- Masters of Misdirection: Peptidoglycan Glycosidases in Bacterial Growth.Journal of bacteriology · 2023Review
- Cell Sorting-Directed Selection of Bacterial Cells in Bigger Sizes Analyzed by Imaging Flow Cytometry during Experimental Evolution.International journal of molecular sciences · 2023Article
- The AmiC/NlpD Pathway Dominates Peptidoglycan Breakdown in Neisseria meningitidis and Affects Cell Separation, NOD1 Agonist Production, and Infection.Infection and immunity · 2022Article
- The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.PLoS genetics · 2022Article
- A lipoprotein allosterically activates the CwlD amidase during Clostridioides difficile spore formation.PLoS genetics · 2021Article
- Understanding tolerance to cell wall-active antibiotics.Annals of the New York Academy of Sciences · 2021Review
- The active repertoire of Escherichia coli peptidoglycan amidases varies with physiochemical environment.Molecular microbiology · 2021Article
- Article
- Vibrio fischeri Amidase Activity Is Required for Normal Cell Division, Motility, and Symbiotic Competence.Applied and environmental microbiology · 2021Article
- Diversification of LytM Protein Functions in Polar Elongation and Cell Division ofFrontiers in microbiology · 2021Article
- Review
- Structural basis of peptidoglycan endopeptidase regulation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Peptidoglycan Amidase Activator Impacts Salmonella enterica Serovar Typhimurium Gut Infection.Infection and immunity · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Synthesis and hydrolysis of septal peptidoglycan (PG) are critical processes at the conclusion of cell division that enable separation of daughter cells. Cleavage of septal PG is mediated by PG amidases, hydrolytic enzymes that release peptide side chains from the glycan strand. Most gammaproteobacteria, including Escherichia coli, encode several functionally redundant periplasmic amidases. However, members of the Vibrio genus, including the enteric pathogen Vibrio cholerae, encode only a single PG amidase, AmiB. Here, we show that V. cholerae AmiB is crucial for cell division and growth. Genetic and biochemical analyses indicated that AmiB is regulated by two activators, EnvC and NlpD, at least one of which is required for AmiB's localization to the cell division site. Localization of the activators (and thus of AmiB) is dependent upon the cell division protein FtsN. These factors mediate septal PG cleavage in E. coli as well; however, their precise roles vary between the two organisms in a number of ways. Notably, even though V. cholerae EnvC and NlpD appear to be functionally redundant under most growth conditions tested, NlpD is specifically required for intestinal colonization in the infant mouse model of cholera and for V. cholerae resistance against bile salts, perhaps due to environmental regulation of AmiB or its activators. Collectively, our findings reveal that although the cellular components that enable cleavage of septal PG appear to be generally conserved between E. coli and V. cholerae, they can be combined into diverse functional regulatory networks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.