ArticleJournal of bacteriology2014
Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 71 citations in OpenAlex.
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- Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling.International journal of molecular sciences · 2023Article
- Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations.Biomolecules · 2023Article
- Community science designed ribosomes with beneficial phenotypes.Nature communications · 2023Article
- Magnesium Modulates Bacillus subtilis Cell Division Frequency.Journal of bacteriology · 2023Article
- Physiological importance and role of MgFrontiers in microbiology · 2023Article
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- Combined proteomic and transcriptomic analysis of the antimicrobial mechanism of tannic acid againstFrontiers in pharmacology · 2023Article
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- A magnesium transporter is involved in the cesium ion resistance of the high-concentration cesium ion-resistant bacteriumFrontiers in microbiology · 2023Article
- Loss of CorA, the primary magnesium transporter of Salmonella, is alleviated by MgtA and PhoP-dependent compensatory mechanisms.PloS one · 2023Article
- Article
- Evolutionary paths to macrolide resistance in a Neisseria commensal converge on ribosomal genes through short sequence duplications.PloS one · 2022Article
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9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To elucidate the biological functions of the ribosomal protein L34, which is encoded by the rpmH gene, the rpmH deletion mutant of Bacillus subtilis and two suppressor mutants were characterized. Although the ΔrpmH mutant exhibited a severe slow-growth phenotype, additional mutations in the yhdP or mgtE gene restored the growth rate of the ΔrpmH strain. Either the disruption of yhdP, which is thought to be involved in the efflux of Mg(2+), or overexpression of mgtE, which plays a major role in the import of Mg(2+), could suppress defects in both the formation of the 70S ribosome and growth caused by the absence of L34. Interestingly, the Mg(2+) content was lower in the ΔrpmH cells than in the wild type, and the Mg(2+) content in the ΔrpmH cells was restored by either the disruption of yhdP or overexpression of mgtE. In vitro experiments on subunit association demonstrated that 50S subunits that lacked L34 could form 70S ribosomes only at a high concentration of Mg(2+). These results showed that L34 is required for efficient 70S ribosome formation and that L34 function can be restored partially by Mg(2+). In addition, the Mg(2+) content was consistently lower in mutants that contained significantly reduced amounts of the 70S ribosome, such as the ΔrplA (L1) and ΔrplW (L23) strains and mutant strains with a reduced number of copies of the rrn operon. Thus, the results indicated that the cellular Mg(2+) content is influenced by the amount of 70S ribosomes.
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