ArticleJournal of bacteriology2014
Bicarbonate increases binding affinity of Vibrio cholerae ToxT to virulence gene promoters.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Strain-dependentFrontiers in microbiology · 2026Article
- Article
- Advances in cholera research: from molecular biology to public health initiatives.Frontiers in microbiology · 2023Review
- Bile Salts Promote ToxR Regulon Activation during Growth under Virulence-Inducing Conditions.Infection and immunity · 2021Article
- H-NS and ToxT Inversely Control Cholera Toxin Production by Binding to Overlapping DNA Sequences.Journal of bacteriology · 2021Article
- Benzoxaboroles: New Potent Inhibitors of the Carbonic Anhydrases of the Pathogenic BacteriumACS medicinal chemistry letters · 2020Article
- Siderophore piracy enhancesMicrobiology (Reading, England) · 2020Article
- Vibrio cholerae Virulence Activator ToxR Regulates Manganese Transport and Resistance to Reactive Oxygen Species.Infection and immunity · 2020Article
- Cholera Toxin Production inFrontiers in microbiology · 2020Article
- Virulence Regulation and Innate Host Response in the Pathogenicity ofFrontiers in cellular and infection microbiology · 2020Review
- Bacillus anthracis Virulence Regulator AtxA Binds Specifically to theJournal of bacteriology · 2019Article
- Seeking new approach for therapeutic treatment of cholera disease via inhibition of bacterial carbonic anhydrases: experimental and theoretical studies for sixteen benzenesulfonamide derivatives.Journal of enzyme inhibition and medicinal chemistry · 2019Article
- Regulated Proteolysis inFrontiers in cellular and infection microbiology · 2019Review
- Synthesis of novel benzenesulfamide derivatives with inhibitory activity against human cytosolic carbonic anhydrase I and II and Vibrio cholerae α- and β-class enzymes.Journal of enzyme inhibition and medicinal chemistry · 2018Article
- OxyR-activated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis.PloS one · 2017Article
- Vibrio cholerae Biofilms and Cholera Pathogenesis.PLoS neglected tropical diseases · 2016Review
- [Bio-protocol · 2015Article
- Conjugated Linoleic Acid Reduces Cholera Toxin Production In Vitro and In Vivo by Inhibiting Vibrio cholerae ToxT Activity.Antimicrobial agents and chemotherapy · 2015Article
- Mechanism for inhibition of Vibrio cholerae ToxT activity by the unsaturated fatty acid components of bile.Journal of bacteriology · 2015Article
- Intestinal Colonization Dynamics of Vibrio cholerae.PLoS pathogens · 2015Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The major Vibrio cholerae virulence gene transcription activator, ToxT, is responsible for the production of the diarrhea-inducing cholera toxin (CT) and the major colonization factor, toxin coregulated pilus (TCP). In addition to the two primary virulence factors mentioned, ToxT is responsible for the activation of accessory virulence genes, such as aldA, tagA, acfA, acfD, tcpI, and tarAB. ToxT activity is negatively modulated by bile and unsaturated fatty acids found in the upper small intestine. Conversely, previous work identified another intestinal signal, bicarbonate, which enhances the ability of ToxT to activate production of CT and TCP. The work presented here further elucidates the mechanism for the enhancement of ToxT activity by bicarbonate. Bicarbonate was found to increase the activation of ToxT-dependent accessory virulence promoters in addition to those that produce CT and TCP. Bicarbonate is taken up into the V. cholerae cell, where it positively affects ToxT activity by increasing DNA binding affinity for the virulence gene promoters that ToxT activates regardless of toxbox configuration. The increase in ToxT binding affinity in the presence of bicarbonate explains the elevated level of virulence gene transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.