ArticleRedox biology2014
Oxidative damage in the gastrocnemius of patients with peripheral artery disease is myofiber type selective.
Article in Redox biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 117 citations in OpenAlex.
- Cocoa flavanols, Nrf2 activation, and oxidative stress in peripheral artery disease: mechanistic findings in muscle based on outcomes from a randomized trial.American journal of physiology. Cell physiology · 2024Trial
- Spatially and temporally resolved assessment of quantitative skeletal muscle perfusion with non-contrast MRI in patients with PAD and healthy controls.Quantitative imaging in medicine and surgery · 2026Article
- Article
- Differential Myopathic and Transcriptomic Changes in Soleus and Gastrocnemius Muscles in a Novel Chronic Hindlimb Ischemia Rat Model Induced by Endovascular Catheter Occlusion.Acta physiologica (Oxford, England) · 2026Article
- Cilostazol use and calf muscle pathophysiology in people with peripheral artery disease.Vascular medicine (London, England) · 2026Article
- Gastrocnemius Myofiber Type and Mitochondrial Alterations Associated With Peripheral Artery Disease Severity.Function (Oxford, England) · 2025Article
- Deciphering the Role of Ferroptosis in the Pathogenesis of Peripheral Artery Disease Myopathy.Biology · 2025Article
- Chronic alcohol consumption exacerbates ischemia-associated skeletal muscle mitochondrial dysfunction in a murine model of peripheral artery disease.Biochimica et biophysica acta. Molecular basis of disease · 2025Article
- Circadian Dysfunction in the Skeletal Muscle Impairs Limb Perfusion and Muscle Regeneration in Peripheral Artery Disease.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- ICAM1 blockade improves ischemic muscle reperfusion in diabetic mice.Cardiovascular diabetology · 2025Article
- Telmisartan modulates exercise responses in peripheral artery disease: Analyses of skeletal muscle from the TELEX Trial.JVS-vascular science · 2025Article
- Skeletal muscle desmin alterations following revascularization in peripheral artery disease claudicants.Scientific reports · 2024Article
- Multi-modality imaging for assessment of the microcirculation in peripheral artery disease: Bench to clinical practice.American heart journal plus : cardiology research and practice · 2024Article
- Clinical physiology: the crucial role of MRI in evaluation of peripheral artery disease.American journal of physiology. Heart and circulatory physiology · 2024Review
- Oxidative damage in the gastrocnemius predicts long-term survival in patients with peripheral artery disease.npj aging · 2024Article
- Linking myosin heavy chain isoform shift to mechanical properties and fracture modes in skeletal muscle tissue.Biomechanics and modeling in mechanobiology · 2024Article
- Mitochondrial fatty acid beta-oxidation: a possible therapeutic target for skeletal muscle lipotoxicity in peripheral artery disease myopathy.EXCLI journal · 2024Review
- Transcriptomic Profiling Identifies Ferroptosis-Related Gene Signatures in Ischemic Muscle Satellite Cells Affected by Peripheral Artery Disease-Brief Report.Arteriosclerosis, thrombosis, and vascular biology · 2023Article
- Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease.Translational research : the journal of laboratory and clinical medicine · 2023Article
- Skeletal Muscle Bioenergetics in Critical Limb Ischemia and Diabetes.The Journal of surgical research · 2023Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPeripheral artery disease (PAD), a manifestation of systemic atherosclerosis that produces blockages in the arteries supplying the legs, affects approximately 5% of Americans. We have previously, demonstrated that a myopathy characterized by myofiber oxidative damage and degeneration is central to PAD pathophysiology.
objectivesIn this study, we hypothesized that increased oxidative damage in the myofibers of the gastrocnemius of PAD patients is myofiber-type selective and correlates with reduced myofiber size.
methodsNeedle biopsies were taken from the gastrocnemius of 53 PAD patients (28 with early PAD and 25 with advanced PAD) and 25 controls. Carbonyl groups (marker of oxidative damage), were quantified in myofibers of slide-mounted tissue, by quantitative fluorescence microscopy. Myofiber cross-sectional area was determined from sarcolemma labeled with wheat germ agglutinin. The tissues were also labeled for myosin I and II, permitting quantification of oxidative damage to and relative frequency of the different myofiber Types (Type I, Type II and mixed Type I/II myofibers). We compared PAD patients in early (N=28) vs. advanced (N=25) disease stage for selective, myofiber oxidative damage and altered morphometrics.
resultsThe carbonyl content of gastrocnemius myofibers was higher in PAD patients compared to control subjects, for all three myofiber types (p<0.05). In PAD patients carbonyl content was higher (p<0.05) in Type II and I/II fibers compared to Type I fibers. Furthermore, the relative frequency and cross-sectional area of Type II fibers were lower, while the relative frequencies and cross-sectional area of Type I and Type I/II fibers were higher, in PAD compared to control gastrocnemius (p<0.05). Lastly, the type II-selective oxidative damage increased and myofiber size decreased as the disease progressed from the early to advanced stage.
conclusionsOur data confirm increased myofiber oxidative damage and reduced myofiber size in PAD gastrocnemius and demonstrate that the damage is selective for type II myofibers and is worse in the most advanced stage of PAD.
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