ArticleRedox biology2014
Pro-oxidant activity of indicaxanthin from Opuntia ficus indica modulates arachidonate metabolism and prostaglandin synthesis through lipid peroxide production in LPS-stimulated RAW 264.7 macrophages.
Article in Redox biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 49 citations in OpenAlex.
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- Impact of Alcalase Hydrolysis and Simulated Gastrointestinal Digestion on the Release of Bioactive Peptides fromInternational journal of molecular sciences · 2024Article
- Ultrasound-assisted extraction and encapsulation of betalain from prickly pear: Process optimization, in-vitro digestive stability, and development of functional gummies.Ultrasonics sonochemistry · 2024Article
- A Combination of Polymethoxyflavones fromAntioxidants (Basel, Switzerland) · 2023Article
- Molecular and Antioxidant Characterization ofAntioxidants (Basel, Switzerland) · 2023Article
- Redox Properties, Bioactivity and Health Effects of Indicaxanthin, a Bioavailable Phytochemical fromAntioxidants (Basel, Switzerland) · 2022Review
- Indicaxanthin fromAntioxidants (Basel, Switzerland) · 2021Article
- Biological Properties and Applications of Betalains.Molecules (Basel, Switzerland) · 2021Review
- Phytochemical Molecules from the Decarboxylation of Gomphrenins in VioletInternational journal of molecular sciences · 2020Article
- Anti-Proliferative Activity of A Hydrophilic Extract of Manna fromMolecules (Basel, Switzerland) · 2020Article
- The Phytochemical Indicaxanthin Synergistically Enhances Cisplatin-Induced Apoptosis in HeLa Cells via Oxidative Stress-Dependent p53/p21Biomolecules · 2020Article
- Cytotoxic Activity of Organotin(IV) Derivatives with Triazolopyrimidine Containing Exocyclic Oxygen Atoms.Molecules (Basel, Switzerland) · 2020Article
- Indicaxanthin fromOxidative medicine and cellular longevity · 2019Article
- Short-term cactus pear [Food & nutrition research · 2018Article
- Brain Distribution and Modulation of Neuronal Excitability by Indicaxanthin FromFrontiers in aging neuroscience · 2018Article
- Effects of gardenia seed, green tea, and cactus pear in rice batter on the chemical quality of lotus rootFood science and biotechnology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Macrophages come across active prostaglandin (PG) metabolism during inflammation, shunting early production of pro-inflammatory towards anti-inflammatory mediators terminating the process. This work for the first time provides evidence that a phytochemical may modulate the arachidonate (AA) metabolism in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, promoting the ultimate formation of anti-inflammatory cyclopentenone 15deoxy-PGJ2. Added 1 h before LPS, indicaxanthin from Opuntia Ficus Indica prevented activation of nuclear factor-κB (NF-κB) and over-expression of PGE2 synthase-1 (mPGES-1), but up-regulated cyclo-oxygenase-2 (COX-2) and PGD2 synthase (H-PGDS), with final production of the anti-inflammatory cyclopentenone. The effects were positively related with concentration between 50 and 100 µM. Indicaxanthin did not have any effect in the absence of LPS. A kinetic study investigating the redox status of LPS-stimulated macrophages between 0.5 and 12 h, either in the absence or in the presence of 50-100 µM indicaxanthin, revealed a differential control of ROS production, with early (0.5-3 h) modest inhibition, followed by a progressive (3-12 h) concentration-dependent enhancement over the level induced by LPS alone. In addition, indicaxanthin caused early (0.5-3 h) concentration-dependent elevation of conjugated diene lipid hydroperoxides, and production of hydroxynonenal-protein adducts, over the amount induced by LPS. In LPS-stimulated macrophages indicaxanthin did not affect PG metabolism when co-incubated with either an inhibitor of NADPH oxidase or vitamin E. It is concluded that LPS-induced pro-oxidant activity of indicaxanthin at the membrane level allows formation of signaling intermediates whose accumulation modulates PG biosynthetic pathway in inflamed macrophages.
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