ArticleDevelopmental biology2014
An arterial-specific enhancer of the human endothelin converting enzyme 1 (ECE1) gene is synergistically activated by Sox17, FoxC2, and Etv2.
Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- Cardiovascular Organoids With Adjustable Endothelial Composition via SOX17-Engineered hPSCs.Biotechnology and bioengineering · 2026Article
- An Integrated Cardiac Microtissue Proteome Map Extends Therapeutic Remodeling by Nanovesicles.Molecular & cellular proteomics : MCP · 2026Article
- Regulation of endothelial cell chromatin availability and transcription factor activity during arterial-venous specification.Development (Cambridge, England) · 2026Article
- Article
- Transcription factors regulating vasculogenesis and angiogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Review
- Transcriptional regulators of arterial and venous identity in the developing mammalian embryo.Current opinion in physiology · 2023Review
- Single cell evaluation of endocardial Hand2 gene regulatory networks reveals HAND2-dependent pathways that impact cardiac morphogenesis.Development (Cambridge, England) · 2023Article
- ETV2 and VEZF1 interaction and regulation of the hematoendothelial lineage during embryogenesis.Frontiers in cell and developmental biology · 2023Article
- GATA4 Regulates Developing Endocardium Through Interaction With ETS1.Circulation research · 2022Article
- Endothelial Cell Differentiation and Hemogenic Specification.Cold Spring Harbor perspectives in medicine · 2022Review
- A SOX17-PDGFB signaling axis regulates aortic root development.Nature communications · 2022Article
- ETS factors are required but not sufficient for specific patterns of enhancer activity in different endothelial subtypes.Developmental biology · 2021Article
- Importance of endothelial Hey1 expression for thoracic great vessel development and its distal enhancer for Notch-dependent endothelial transcription.The Journal of biological chemistry · 2020Article
- Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart.Nature communications · 2019Article
- Foxc2 is required for proper cardiac neural crest cell migration, outflow tract septation, and ventricle expansion.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Article
- Transcriptional and epigenomic landscapes of CNS and non-CNS vascular endothelial cells.eLife · 2018Article
- Etv2 as an essential regulator of mesodermal lineage development.Cardiovascular research · 2017Review
- SoxF factors induce Notch1 expression via direct transcriptional regulation during early arterial development.Development (Cambridge, England) · 2017Article
- Cooperative activation of cardiac transcription through myocardin bridging of paired MEF2 sites.Development (Cambridge, England) · 2017Article
- Pharmacological targeting of the transcription factor SOX18 delays breast cancer in mice.eLife · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Endothelin-converting enzyme-1 (Ece-1), a crucial component of the Endothelin signaling pathway, is required for embryonic development and is an important regulator of vascular tone, yet the transcriptional regulation of the ECE1 gene has remained largely unknown. Here, we define the activity and regulation of an enhancer from the human ECE1 locus in vivo. The enhancer identified here becomes active in endothelial progenitor cells shortly after their initial specification and is dependent on a conserved FOX:ETS motif, a composite binding site for Forkhead transcription factors and the Ets transcription factor Etv2, for activity in vivo. The ECE1 FOX:ETS motif is bound and cooperatively activated by FoxC2 and Etv2, but unlike other described FOX:ETS-dependent enhancers, ECE1 enhancer activity becomes restricted to arterial endothelium and endocardium by embryonic day 9.5 in transgenic mouse embryos. The ECE1 endothelial enhancer also contains an evolutionarily-conserved, consensus SOX binding site, which is required for activity in transgenic mouse embryos. Importantly, the ECE1 SOX site is bound and activated by Sox17, a transcription factor involved in endothelial cell differentiation and an important regulator of arterial identity. Moreover, the ECE1 enhancer is cooperatively activated by the combinatorial action of FoxC2, Etv2, and Sox17. Although Sox17 is required for arterial identity, few direct transcriptional targets have been identified in endothelial cells. Thus, this work has important implications for our understanding of endothelial specification and arterial subspecification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.