Evidence map›Paper›PMID 25178952›Full record

ArticleNature communications2014

TRPV channel-mediated calcium transients in nociceptor neurons are dispensable for avoidance behaviour.

Amanda S Lindy, Puja K Parekh, Richard Zhu, Patrick Kanju, Sree V Chintapalli, Volodymyr Tsvilovskyy, Randen L Patterson, Andriy Anishkin, Damian B van Rossum, Wolfgang B Liedtke

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.0field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. TRPV3: Structure, Diseases and Modulators.Molecules (Basel, Switzerland) · 2023
    Review
  4. TRPV4: A trigger of pathological RhoA activation in neurological disease.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022
    Article
  5. Reduced CaFrontiers in neuroscience · 2022
    Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 2 countries.

Amanda S Lindy1] Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA [2] Department of Neurology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Puja K ParekhDepartment of Neurology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Richard ZhuDepartment of Neurology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Patrick KanjuDepartment of Neurology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Sree V Chintapalli1] Department of Membrane Biology and Physiology, University of California, Davis, California 95616, USA [2] Department of Biochemistry and Molecular Medicine, University of California, Davis, California 95616, USA.
Volodymyr TsvilovskyyDepartment of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany.
Randen L Patterson1] Department of Membrane Biology and Physiology, University of California, Davis, California 95616, USA [2] Department of Biochemistry and Molecular Medicine, University of California, Davis, California 95616, USA.
Andriy Anishkin1] Center for Computational Proteomics, The Pennsylvania State University, University Park, Pennsylvania 16801, USA [2] Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16801, USA.
Damian B van Rossum1] Center for Computational Proteomics, The Pennsylvania State University, University Park, Pennsylvania 16801, USA [2] Department of Biology, The Pennsylvania State University, University Park, Pennsylvania 16801, USA.
Wolfgang B Liedtke1] Department of Neurology, Duke University Medical Center, Durham, North Carolina 27710, USA [2] Duke University Clinics for Pain and Palliative Care, 932 Morreene Road, Durham, North Carolina 27705, USA [3] Departments of Anesthesiology and Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA [4] Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Duke Medical Center · USDuke University · USPennsylvania State University · USUniversity of California, Davis · USDuke University Hospital · USHeidelberg University · DE

Funding

Detecting Homology in the "Twilight Zone" of Sequence SimilarityR01GM087410 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI PATTERSON, RANDEN LEE · 2009 to 2012
$1.0M
NIGMS NIH HHS R01 GM087410NIGMS NIH HHS R01 GM087410-01
6 · The paper itself

Abstract

Animals need to sense and react to potentially dangerous environments. TRP ion channels participate in nociception, presumably via Ca(2+) influx, in most animal species. However, the relationship between ion permeation and animals' nocifensive behaviour is unknown. Here we use an invertebrate animal model with relevance for mammalian pain. We analyse the putative selectivity filter of OSM-9, a TRPV channel, in osmotic avoidance behaviour of Caenorhabditis elegans. Using mutagenized OSM-9 expressed in the head nociceptor neuron, ASH, we study nocifensive behaviour and Ca(2+) influx. Within the selectivity filter, M(601)-F(609), Y604G strongly reduces avoidance behaviour and eliminates Ca(2+) transients. Y604F also abolishes Ca(2+) transients in ASH, while sustaining avoidance behaviour, yet it disrupts behavioral plasticity. Homology modelling of the OSM-9 pore suggests that Y(604) may assume a scaffolding role. Thus, aromatic residues in the OSM-9 selectivity filter are critical for pain behaviour and ion permeation. These findings have relevance for understanding evolutionary roots of mammalian nociception.

Indexed as

Amino Acid SequenceAmino Acid SubstitutionAnimalsAvoidance LearningCaenorhabditis elegansCaenorhabditis elegans ProteinsCalciumCalcium SignalingGene ExpressionIon TransportModels, MolecularMolecular Sequence DataMutationNerve Tissue ProteinsNociceptionNociceptorsCaenorhabditis elegans ProteinsCalciumNerve Tissue ProteinsOSM-9 protein, C elegansRecombinant Fusion ProteinsTRPV Cation Channels

Identifiers

PMID25178952
PMCPMC4164786
OpenAlexW2087385036

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.