Evidence map›Paper›PMID 25178041›Full record

ArticleChemical communications (Cambridge, England)2014

Chemical biology of genomic DNA: minimizing PCR bias.

Gordon R McInroy, Eun-Ang Raiber, Shankar Balasubramanian

Open access · hybridAbstract read
In one paragraph

Article in Chemical communications (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Metagenomic Analysis of Silage.Journal of visualized experiments : JoVE · 2017
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Gordon R McInroyDepartment of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. sb10031@cam.ac.uk.
Eun-Ang Raiber
Shankar Balasubramanian
University of Cambridge · GB

Funding

Cancer Research UKWellcome Trust
6 · The paper itself

Abstract

The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.

Indexed as

Real-Time Polymerase Chain ReactionAldehydesBiotinylationDNADNA AdductsDNA ProbesHydrogen-Ion ConcentrationOxidation-ReductionAldehydesDNADNA AdductsDNA Probes

Identifiers

PMID25178041
PMCPMC5759930
OpenAlexW1979944115

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.