ArticleJournal of visualized experiments : JoVE2014
Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- The Therapeutic Potential of Pericytes in Bone Tissue Regeneration.Biomedicines · 2023Review
- Article
- Pericytes as a Source of Osteogenic Cells in Bone Fracture Healing.International journal of molecular sciences · 2019Article
- Simultaneous isolation of mesenchymal stem cells and endothelial progenitor cells derived from murine bone marrow.Experimental and therapeutic medicine · 2018Article
- Adipose-derived Human Perivascular Stem Cells May Improve Achilles Tendon Healing in Rats.Clinical orthopaedics and related research · 2018Article
- Type 2 diabetes impairs the ability of skeletal muscle pericytes to augment postischemic neovascularization in db/db mice.American journal of physiology. Cell physiology · 2018Article
- Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement.Journal of visualized experiments : JoVE · 2017Article
- Exploring pericyte and cardiac stem cell secretome unveils new tactics for drug discovery.Pharmacology & therapeutics · 2017Review
- Article
- Mesenchymal Stem Cells Subpopulations: Application for Orthopedic Regenerative Medicine.Stem cells international · 2016Review
- Cultured Human Adipose Tissue Pericytes and Mesenchymal Stromal Cells Display a Very Similar Gene Expression Profile.Stem cells and development · 2015Article
- Development of mRuby2-Transfected C3H10T1/2 Fibroblasts for Musculoskeletal Tissue Engineering.PloS one · 2015Article
- Regenerative Translation of Human Blood-Vessel-Derived MSC Precursors.Stem cells international · 2015Review
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 5 countries.
Funding
Abstract
Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization of MSCs have been obscured by their retrospective isolation in culture. Recently, using fluorescence-activated cell sorting (FACS), we and other researchers prospectively identified and purified three subpopulations of multipotent precursor cells associated with the vasculature of human skeletal muscle. These three cell populations: myogenic endothelial cells (MECs), pericytes (PCs), and adventitial cells (ACs), are localized respectively to the three structural layers of blood vessels: intima, media, and adventitia. All of these human blood-vessel-derived stem cell (hBVSC) populations not only express classic MSC markers but also possess mesodermal developmental potentials similar to typical MSCs. Previously, MECs, PCs, and ACs have been isolated through distinct protocols and subsequently characterized in separate studies. The current isolation protocol, through modifications to the isolation process and adjustments in the selective cell surface markers, allows us to simultaneously purify all three hBVSC subpopulations by FACS from a single human muscle biopsy. This new method will not only streamline the isolation of multiple BVSC subpopulations but also facilitate future clinical applications of hBVSCs for distinct therapeutic purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.