ArticleFrontiers in plant science2014
Autophagy-like processes are involved in lipid droplet degradation in Auxenochlorella protothecoides during the heterotrophy-autotrophy transition.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 58 citations in OpenAlex.
- Review
- Evolutionary conservation and metabolic significance of autophagy in algae.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Growth and Cell Size of MicroalgaMicroorganisms · 2024Article
- Green microalgaFrontiers in microbiology · 2024Article
- CALEOSIN 1 interaction with AUTOPHAGY-RELATED PROTEIN 8 facilitates lipid droplet microautophagy in seedlings.Plant physiology · 2023Article
- Lipid droplets are versatile organelles involved in plant development and plant response to environmental changes.Frontiers in plant science · 2023Review
- Distinct roles of alternative oxidase pathway during the greening process of etiolated algae.Science China. Life sciences · 2021Article
- Autophagy is required for lipid homeostasis during dark-induced senescence.Plant physiology · 2021Article
- Reserve lipids and plant autophagy.Journal of experimental botany · 2020Review
- The MicroalgaPlant physiology · 2020Article
- Genomic Characterization and Expressional Profiles of Autophagy-Related Genes (International journal of molecular sciences · 2020Article
- Degradation of Lipid Droplets in Plants and Algae-Right Time, Many Paths, One Goal.Frontiers in plant science · 2020Review
- Lipidome Remodeling and Autophagic Respose in the Arachidonic-Acid-Rich MicroalgaFrontiers in plant science · 2020Article
- Inhibition of glucose assimilation inBiotechnology for biofuels · 2020Article
- Article
- Dual Role for Autophagy in Lipid Metabolism in Arabidopsis.The Plant cell · 2019Article
- Possible Roles of Membrane Trafficking Components for Lipid Droplet Dynamics in Higher Plants and Green Algae.Frontiers in plant science · 2019Review
- Autophagy-Mediated Regulation of Lipid Metabolism and Its Impact on the Growth in Algae and Seed Plants.Frontiers in plant science · 2019Review
- Chloroplast Damage Induced by the Inhibition of Fatty Acid Synthesis Triggers Autophagy in Chlamydomonas.Plant physiology · 2018Article
- PUX10 Is a Lipid Droplet-Localized Scaffold Protein That Interacts with CELL DIVISION CYCLE48 and Is Involved in the Degradation of Lipid Droplet Proteins.The Plant cell · 2018Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Autophagy is a cellular degradation process that recycles cytoplasmic components in eukaryotes. Although intensively studied in yeast, plants, and mammals, autophagy in microalgae is not well understood. Auxenochlorella protothecoides is a green microalga that has the ability to grow either autotrophically when under light or heterotrophically when in media containing glucose. The two growth modes are inter-convertible and transition between them is accompanied by drastic changes in morphology and cellular composition; however, the mechanisms underlying these changes are unknown. In this study, we identified autophagy-related genes and characterized their roles in the degradation of lipid droplets during the heterotrophy-to-autotrophy (HA) transition in A. protothecoides. Most of the proteins constituting the eukaryotic "core machinery" were conserved in A. protothecoides. Two proteins, Atg4 and Atg8, were further investigated. A. protothecoides ATG4 was cloned from a cDNA library and expressed within yeast, and was able to functionally restore the autophagy pathway in atg4Δ yeast during nitrogen starvation. Furthermore, Atg8, which displayed high sequence identity with its yeast homolog, was able to conjugate to phosphatidylethanolamine (PE) in vitro and was recruited to the phagophore assembly site in yeast. We also identified a C-terminal glycine residue, G118, that was the cleavage site for Atg4. Finally, we used confocal and transmission electron microscopy to reveal that autophagic-like vacuoles were detectable in algal cells during the HA transition. Our data suggested that the lipid droplets in heterotrophic cells were engulfed directly by the autophagic-like vacuole instead of via autophagosomes.
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