ArticleBMC infectious diseases2014
Automated image-based assay for evaluation of HIV neutralization and cell-to-cell fusion inhibition.
Article in BMC infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Galectin-8 binds HIV envelope glycoproteins with high affinity and promotes viral infectivity.Frontiers in cellular and infection microbiology · 2026Article
- Scaling up delivery of HIV services in Africa through harnessing trends across global emerging innovations.Frontiers in health services · 2023Review
- HIV-2 Neutralization Sensitivity in Relation to Co-Receptor Entry Pathways and Env Motifs.International journal of molecular sciences · 2022Article
- Where in the Cell Are You? Probing HIV-1 Host Interactions through Advanced Imaging Techniques.Viruses · 2016Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundStandardized techniques to detect HIV-neutralizing antibody responses are of great importance in the search for an HIV vaccine.
methodsHere, we present a high-throughput, high-content automated plaque reduction (APR) assay based on automated microscopy and image analysis that allows evaluation of neutralization and inhibition of cell-cell fusion within the same assay. Neutralization of virus particles is measured as a reduction in the number of fluorescent plaques, and inhibition of cell-cell fusion as a reduction in plaque area.
resultsWe found neutralization strength to be a significant factor in the ability of virus to form syncytia. Further, we introduce the inhibitory concentration of plaque area reduction (ICpar) as an additional measure of antiviral activity, i.e. fusion inhibition.
conclusionsWe present an automated image based high-throughput, high-content HIV plaque reduction assay. This allows, for the first time, simultaneous evaluation of neutralization and inhibition of cell-cell fusion within the same assay, by quantifying the reduction in number of plaques and mean plaque area, respectively. Inhibition of cell-to-cell fusion requires higher quantities of inhibitory reagent than inhibition of virus neutralization.
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Registered trials
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