ArticleMolecular carcinogenesis2015
PNA binding to the non-template DNA strand interferes with transcription, suggesting a blockage mechanism mediated by R-loop formation.
Article in Molecular carcinogenesis, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- The CGG triplet repeat binding protein 1 counteracts R-loop induced transcription-replication stress.EMBO reports · 2025Article
- Detecting R-Loop Formation Using a Plasmid-Based In Vitro Transcription Assay.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Topology and kinetics of R-loop formation.Biophysical journal · 2022Article
- R-loop generation during transcription: Formation, processing and cellular outcomes.DNA repair · 2018Review
- The Emerging Role of DNA Damage in the Pathogenesis of the C9orf72 Repeat Expansion in Amyotrophic Lateral Sclerosis.International journal of molecular sciences · 2018Review
- Alternative DNA secondary structure formation affects RNA polymerase II promoter-proximal pausing in human.Genome biology · 2018Article
- A novel mode for transcription inhibition mediated by PNA-induced R-loops with a model in vitro system.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Article
- Strong transcription blockage mediated by R-loop formation within a G-rich homopurine-homopyrimidine sequence localized in the vicinity of the promoter.Nucleic acids research · 2017Article
- When transcription goes on Holliday: Double Holliday junctions block RNA polymerase II transcription in vitro.Biochimica et biophysica acta. Gene regulatory mechanisms · 2017Article
- R-loop: an emerging regulator of chromatin dynamics.Acta biochimica et biophysica Sinica · 2016Review
- Molecular recognition mechanism of peptide chain bound to the tRNA(Lys3) anticodon loop in silico.Journal of biomolecular structure & dynamics · 2015Article
- A peptide nucleic acid targeting nuclear RAD51 sensitizes multiple myeloma cells to melphalan treatment.Cancer biology & therapy · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Peptide Nucleic Acids (PNAs) are artificial DNA mimics with superior nucleic acid binding capabilities. T7 RNA polymerase (T7 RNAP) transcription upon encountering PNA bound to the non-template DNA strand was studied in vitro. A characteristic pattern of blockage signals was observed, extending downstream from the PNA binding site, similar to that produced by G-rich homopurine-homopyrimidine (hPu-hPy) sequences and likely caused by R-loop formation. Since blocked transcription complexes in association with stable R-loops may interfere with replication and in some cases trigger apoptosis, targeted R-loop formation might be employed to inactivate selected cells, such as those in tumors, based upon their unique complement of expressed genes.
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