ArticleActa biomaterialia2014
Novel osteoinductive photo-cross-linkable chitosan-lactide-fibrinogen hydrogels enhance bone regeneration in critical size segmental bone defects.
Article in Acta biomaterialia, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Association between fibrinogen concentration and nonunion in fracture patients.Frontiers in surgery · 2026Article
- Combining a Vascular Bundle and 3D Printed Scaffold with BMP-2 Improves Bone Repair and Angiogenesis.Tissue engineering. Part A · 2021Article
- Regeneration enhanced in critical-sized bone defects using bone-specific extracellular matrix protein.Journal of biomedical materials research. Part B, Applied biomaterials · 2021Article
- Progress and Prospects of Polymer-Based Drug Delivery Systems for Bone Tissue Regeneration.Polymers · 2020Review
- Porous Silk Fibroin/Cellulose Hydrogels for Bone Tissue Engineering via a Novel Combined Process Based on Sequential Regeneration and Porogen Leaching.Molecules (Basel, Switzerland) · 2020Article
- Development prospects of curable osteoplastic materials in dentistry and maxillofacial surgery.Heliyon · 2020Review
- Oral Bone Tissue Engineering: Advanced Biomaterials for Cell Adhesion, Proliferation and Differentiation.Materials (Basel, Switzerland) · 2019Review
- The effect of pulsed electromagnetic field exposure on osteoinduction of human mesenchymal stem cells cultured on nano-TiO2 surfaces.PloS one · 2018Article
- Cell-laden hydrogels for osteochondral and cartilage tissue engineering.Acta biomaterialia · 2017Review
- Vascularization in bone tissue engineering constructs.Annals of biomedical engineering · 2015Review
- Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine.BioMed research international · 2015Review
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Authors and funding
7 authors.
Funding
Abstract
The purpose of this study was to develop and characterize a novel photo-cross-linkable chitosan-lactide-fibrinogen (CLF) hydrogel and evaluate the efficacy of bone morphogenetic protein-2 (BMP-2) containing a CLF hydrogel for osteogenesis in vitro and in vivo. We synthesized the CLF hydrogels and characterized their chemical structure, degradation rate, compressive modulus and in vitro BMP-2 release kinetics. We evaluated bioactivities of the BMP-2 containing CLF hydrogels (0, 50, 100 and 500ngml(-1)) in vitro using W-20-17 preosteoblast mouse bone marrow stromal cells and C2C12 mouse myoblast cells. The effect of BMP-2 containing CLF gels (0, 0.5, 1, 2 and 5μg) on bone formation was evaluated using rat critical size segmental bone defects for 4weeks. Fourier transform infrared spectroscopy spectra and scanning electron microscopy images showed chemical and structural changes by the addition of fibrinogen into the chitosan-lactide copolymer. The incorporation of fibrinogen molecules significantly increased the compressive modulus of the hydrogels. The in vitro BMP-2 release study showed initial burst releases from the CLF hydrogels followed by sustained releases, regardless of the concentration of the BMP-2 over 4weeks. Cells in all groups were viable in the presence of the hydrogels regardless of BMP-2 doses, indicating non-cytotoxicity of hydrogels. Alkaline phosphate activity and mineralization of cells exhibited dose dependence on BMP-2 containing CLF hydrogels. Radiography, microcomputed tomography and histology confirmed that the BMP-2 containing CLF hydrogels prompted neo-osteogenesis and accelerated healing of the defects in a dose-dependent manner. Thus the CLF hydrogel is a promising delivery system of growth factors for bone regeneration.
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