ArticleParasites & vectors2014
Phenotypic variation in Blastocystis sp. ST3.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
- Associations between Blastocystis spp., Campylobacter spp., Giardia duodenalis and Dientamoeba fragilis using Bayesian priors to account for diagnostic test accuracy.European journal of microbiology & immunology · 2025Article
- Gut bacteria influence Blastocystis sp. phenotypes and may trigger pathogenicity.PLoS neglected tropical diseases · 2023Article
- Higher amoebic and metronidazole resistant forms of Blastocystis sp. seen in schizophrenic patients.Parasites & vectors · 2022Article
- Distinct Phenotypic Variation ofBiology · 2022Article
- An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals.Acta parasitologica · 2022Article
- Association ofWorld journal of gastrointestinal oncology · 2022Article
- Blastocystis ST-1 is associated with Irritable Bowel Syndrome-diarrhoea (IBS-D) in Indonesian adolescences.Parasite epidemiology and control · 2019Article
- Surface ultrastructure, protein profile and zymography ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2019Article
- Article
- In vitro and in vivo thermal stress induces proliferation of Blastocystis sp.Parasitology research · 2018Article
- Prevalence and subtype identification ofGastroenterology and hepatology from bed to bench · 2017Article
- Increase number of mitochondrion-like organelle in symptomatic Blastocystis subtype 3 due to metronidazole treatment.Parasitology research · 2016Article
- The distribution of Blastocystis subtypes in isolates from Qatar.Parasites & vectors · 2015Article
- Factors that influence the shedding of Blastocystis cysts in an irritable bowel syndrome (IBS) patient--an evidence-based case study.Parasitology research · 2015Article
- Blastocystis sp. in Irritable Bowel Syndrome (IBS)--Detection in Stool Aspirates during Colonoscopy.PloS one · 2015Article
- Article
- Suitability of internal transcribed spacers (ITS) as markers for the population genetic structure of Blastocystis spp.Parasites & vectors · 2014Article
- Comprehensive Study of Phenotypic and Growth Rate Features ofIranian journal of parasitologyArticle
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4 authors.
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Abstract
backgroundBlastocystis, is one of the most common human intestinal protozoan, which has many conflicting reports on its pathogenic role. Gut conditions which obviously varies in asymptomatic individuals, symptomatic and irritable bowel syndrome (IBS) patients in terms of gut flora, pH, osmotic pressure and water potentials could play an important role in its pathogenicity. The present study is the first study to investigate phenotypic characteristics of Blastocystis sp. ST3 isolated from asymptomatic, symptomatic and IBS isolates.
methodsA total of 8 Blastocystis isolates were obtained from four IBS patients (IBS1-4) and four symptomatic patients (S1-4) at a local gastroenterology clinic. Asymptomatic isolates (A1-4) were obtained from a field survey at a local village.
resultsAll 12 isolates were determined as subtype 3 (ST3). A1-4 isolates showed the highest peak growth followed by IBS1-4 isolates and S1-4 isolates for the growth profile. Parasites from IBS isolates (IBS1-4) showed the largest diameter with a mean 18.43 ± 2.22 μm compared to parasites of symptomatic isolates (isolates S1-4) 15.54 ± 3.02 μm and asymptomatic isolates (isolates A1-4) 11.76 ± 0.82 μm. The symptomatic isolates (average generation time: 9.87 ± 2.97 h) grew faster than the IBS isolates (average generation time: 7.56 ± 1.06 h) and asymptomatic isolates (average generation time: 5.97 ± 1.52 h). The parasites isolated from IBS isolates showed strong aggregation and clumping, which had seen reduced in parasites of isolates S1-4. No clumping was seen in parasites from A1-4. The outer surface of parasites in IBS isolates showed greater binding affinities towards FITC-labelled Concanavalin A (Con A) than symptomatic isolates and asymptomatic isolates. Scanning electron microscopy showed that in IBS isolates, the surface of Blastocystis showed a very coarse and intensely folded surface. The IBS isolates also exhibited a dense material and a thicker layer of surface coat can be seen compared to asymptomatic and symptomatic isolates.
conclusionThere have been no studies thus far providing evidence for phenotypic variation within a particular subtype. The present study is the first to demonstrate the phenomenon of gut environment facilitating adaptation of parasites possibly for survival leading to phenotypic differences for Blastocystis.
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