ArticleJournal of the American Chemical Society2014
Bond-specific dissociation following excitation energy transfer for distance constraint determination in the gas phase.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Mechanisms of SARS-CoV-2 Inactivation Using UVC Laser Radiation.ACS photonics · 2024Article
- Direct Ultraviolet Laser-Induced Reduction of Disulfide Bonds in Insulin and Vasopressin.ACS omega · 2020Article
- Methionine and Selenomethionine as Energy Transfer Acceptors for Biomolecular Structure Elucidation in the Gas Phase.Journal of the American Society for Mass Spectrometry · 2019Article
- Synthesis of New S-S and C-C Bonds by Photoinitiated Radical Recombination Reactions in the Gas Phase.International journal of mass spectrometry · 2019Article
- Simplified identification of disulfide, trisulfide, and thioether pairs with 213 nm UVPD.The Analyst · 2018Article
- Top-Down Characterization of Proteins with Intact Disulfide Bonds Using Activated-Ion Electron Transfer Dissociation.Analytical chemistry · 2018Article
- Combining Structural Probes in the Gas Phase - Ion Mobility-Resolved Action-FRET.Journal of the American Society for Mass Spectrometry · 2018Article
- The Mechanism Behind Top-Down UVPD Experiments: Making Sense of Apparent Contradictions.Journal of the American Society for Mass Spectrometry · 2017Article
- Photoelectron Transfer Dissociation Reveals Surprising Favorability of Zwitterionic States in Large Gaseous Peptides and Proteins.Journal of the American Chemical Society · 2017Article
- Near-UV Photodissociation of Tryptic Peptide Cation Radicals. Scope and Effects of Amino Acid Residues and Radical Sites.Journal of the American Society for Mass Spectrometry · 2017Article
- SITS Derivatization of Peptides to Enhance 266 nm Ultraviolet Photodissociation (UVPD).Journal of the American Society for Mass Spectrometry · 2017Article
- Leveraging Electron Transfer Dissociation for Site Selective Radical Generation: Applications for Peptide Epimer Analysis.Journal of the American Society for Mass Spectrometry · 2017Article
- Action-FRET of a Gaseous Protein.Journal of the American Society for Mass Spectrometry · 2017Article
- Structural Effects of Solvation by 18-Crown-6 on Gaseous Peptides and TrpCage after Electrospray Ionization.Journal of the American Society for Mass Spectrometry · 2016Article
- Characterization of the Cysteine Content in Proteins Utilizing Cysteine Selenylation with 266 nm Ultraviolet Photodissociation (UVPD).Journal of the American Society for Mass Spectrometry · 2016Article
- Enhancing Protein Disulfide Bond Cleavage by UV Excitation and Electron Capture Dissociation for Top-Down Mass Spectrometry.International journal of mass spectrometry · 2015Article
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Authors and funding
5 authors.
Funding
Abstract
Herein, we report chemistry that enables excitation energy transfer (EET) to be accurately measured via action spectroscopy on gaseous ions in an ion trap. It is demonstrated that EET between tryptophan or tyrosine and a disulfide bond leads to excited state, homolytic fragmentation of the disulfide bond. This phenomenon exhibits a tight distance dependence, which is consistent with Dexter exchange transfer. The extent of fragmentation of the disulfide bond can be used to determine the distance between the chromophore and disulfide bond. The chemistry is well suited for the examination of protein structure in the gas phase because native amino acids can serve as the donor/acceptor moieties. Furthermore, both tyrosine and tryptophan exhibit unique action spectra, meaning that the identity of the donating chromophore can be easily determined in addition to the distance between donor/acceptor. Application of the method to the Trpcage miniprotein reveals distance constraints that are consistent with a native-like fold for the +2 charge state in the gas phase. This structure is stabilized by several salt bridges, which have also been observed to be important previously in proteins that retain native-like structures in the gas phase. The ability of this method to measure specific distance constraints, potentially at numerous positions if combined with site-directed mutagenesis, significantly enhances our ability to examine protein structure in the gas phase.
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