ArticleDevelopmental biology2014
Identification of differentially expressed genes during development of the zebrafish pineal complex using RNA sequencing.
Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Transcriptomic signatures of individual cell types in cerebral cavernous malformation.Cell communication and signaling : CCS · 2024Article
- Molecular analyses of zebrafish V0v spinal interneurons and identification of transcriptional regulators downstream of Evx1 and Evx2 in these cells.Neural development · 2023Article
- Mass-spectrometry analysis of the human pineal proteome during night and day and in autism.Journal of pineal research · 2021Article
- Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes.BMC genomics · 2019Article
- Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains.Journal of visualized experiments : JoVE · 2018Article
- A transcription factor network controls cell migration and fate decisions in the developing zebrafish pineal complex.Development (Cambridge, England) · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
We describe a method for isolating RNA suitable for high-throughput RNA sequencing (RNA-seq) from small numbers of fluorescently labeled cells isolated from live zebrafish (Danio rerio) embryos without using costly, commercially available columns. This method ensures high cell viability after dissociation and suspension of cells and gives a very high yield of intact RNA. We demonstrate the utility of our new protocol by isolating RNA from fluorescence activated cell sorted (FAC sorted) pineal complex neurons in wild-type and tbx2b knockdown embryos at 24 hours post-fertilization. Tbx2b is a transcription factor required for pineal complex formation. We describe a bioinformatics pipeline used to analyze differential expression following high-throughput sequencing and demonstrate the validity of our results using in situ hybridization of differentially expressed transcripts. This protocol brings modern transcriptome analysis to the study of small cell populations in zebrafish.
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Registered trials
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