ArticleInsect biochemistry and molecular biology2014
CYP18A1 regulates tissue-specific steroid hormone inactivation in Bombyx mori.
Article in Insect biochemistry and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- MicroRNA novel-miR-90-5p modulates larval molt development in Ostrinia furnacalis (Guenée) by targeting OfCYP18A1.Insect science · 2026Article
- Odorant degrading enzyme candidates enriched in the antennae of the white-spotted flower chafer,Frontiers in insect science · 2026Article
- A-to-I RNA editing ofeLife · 2025Article
- Genome-Wide Profiling of P450 Gene Expression Reveals Caste-Specific and Developmental Patterns inInternational journal of molecular sciences · 2025Article
- Genetic characterization of candidate ecdysteroid kinases in Drosophila melanogaster.G3 (Bethesda, Md.) · 2024Article
- Research Progress on the Regulation of Autophagy and Apoptosis in Insects by Sterol Hormone 20-Hydroxyecdysone.Insects · 2023Review
- Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis.Frontiers in zoology · 2023Article
- Identification of Genes Involved in Resistance to High Exogenous 20-Hydroxyecdysone inInsects · 2022Article
- Article
- Genome-enabled insights into the biology of thrips as crop pests.BMC biology · 2020Article
- Cocoon-Spinning Behavior and 20-Hydroxyecdysone Regulation of Fibroin Genes inFrontiers in physiology · 2020Article
- Identification and Characterization of Genes Involved in Ecdysteroid Esterification Pathway Contributing to the High 20-Hydroxyecdysone Resistance ofFrontiers in physiology · 2020Article
- Regulation of Hormone-Related Genes in Ericerus pela (Hemiptera: Coccidae) for Dimorphic Metamorphosis.Journal of insect science (Online) · 2019Article
- Ecdysone Receptor Agonism Leading to Lethal Molting Disruption in Arthropods: Review and Adverse Outcome Pathway Development.Environmental science & technology · 2017Review
- How Did Arthropod Sesquiterpenoids and Ecdysteroids Arise? Comparison of Hormonal Pathway Genes in Noninsect Arthropod Genomes.Genome biology and evolution · 2015Article
- Ectopic expression of ecdysone oxidase impairs tissue degeneration in Bombyx mori.Proceedings. Biological sciences · 2015Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
Insect development and metamorphosis are regulated by two major hormones, juvenile hormone and ecdysteroids. Despite being the key regulator of insect developmental transitions, the metabolic pathway of the primary steroid hormone, 20-hydroxyecdysone (20E), especially its inactivation pathway, is still not completely elucidated. A cytochrome P450 enzyme, CYP18A1, has been shown to play key roles in insect steroid hormone inactivation through 26-hydroxylation. Here, we identified two CYP18 (BmCYP18A1 and BmCYP18B1) orthologs in the lepidopteran model insect, Bombyx mori. Interestingly, BmCYP18A1 gene is predominantly expressed in the middle silk gland (MSG) while BmCYP18B1 expresses ubiquitously in B. mori. BmCYP18A1 is induced by 20E in vitro, suggesting its role in 20E metabolism. Using the binary Gal4/UAS transgenic system, we ectopically overexpressed BmCYP18A1 in a MSG-specific manner with a Sericin1-Gal4 (Ser-Gal4) driver or in a ubiquitous manner with an Actin3-Gal4 (A3-Gal4) driver. Ectopic overexpression of BmCYP18A1 in MSG or in all tissues resulted in developmental arrestment of transgenic animals during the final instar larval stage. The 20E titers in the transgenic animals expressing BmCYP18A1 were lower compared to the levels in the control animals. Although the biological significance of MSG-specific expression of BmCYP18A1 is unclear, our results provide the first evidence that BmCYP18A1, which is conserved in most arthropods, is involved in a tissue-specific steroid hormone inactivation in B. mori.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.