Evidence map›Paper›PMID 25173582›Full record

ArticleNeuro-oncology2015

Targeting the SMO oncogene by miR-326 inhibits glioma biological behaviors and stemness.

Wenzhong Du, Xing Liu, Lingchao Chen, Zhijin Dou, Xuhui Lei, Liang Chang, Jinquan Cai, Yuqiong Cui, Dongbo Yang, Ying Sun and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuro-oncology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed, 1 pooled it
4.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 1 synthesis or guideline pooled it, 93 citations in OpenAlex.

  1. Pooled it
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  8. Long Non-Coding RNAIranian journal of public health · 2024
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  16. Expression of microRNAs miR-21 and miR-326 associated with HIF-1α regulation in neurospheres of glioblastoma submitted to ionizing radiation treatment.Reports of practical oncology and radiotherapy : journal of Greatpoland Cancer Center in Poznan and Polish Society of Radiation Oncology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Wenzhong DuDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Xing LiuDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Lingchao ChenDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Zhijin DouDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Xuhui LeiDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Liang ChangDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Jinquan CaiDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Yuqiong CuiDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Dongbo YangDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Ying SunDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Yongli LiDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Chuanlu JiangDepartment of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China (W.D., X.L., Z.D., X.L., L.C., J.C., Y.C., D.Y., Y.S., Y.L., C.J.); Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China (L.C.).
Huashan Hospital · CNSecond Affiliated Hospital of Harbin Medical University · CNFudan University · CNHarbin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFew studies have associated microRNAs (miRNAs) with the hedgehog (Hh) pathway. Here, we investigated whether targeting smoothened (SMO) with miR-326 would affect glioma biological behavior and stemness.

methodsTo investigate the expression of SMO and miR-326 in glioma specimens and cell lines, we utilized quantitative real-time (qRT)-PCR, Western blot, immunohistochemistry, and fluorescence in situ hybridization. The luciferase reporter assay was used to verify the relationship between SMO and miR-326. We performed cell counting kit-8, transwell, and flow cytometric assays using annexin-V labeling to detect changes after transfection with siRNA against SMO or miR-326. qRT-PCR assays, neurosphere formation, and immunofluorescence were utilized to detect the modification of self-renewal and stemness in U251 tumor stem cells. A U251-implanted intracranial model was used to study the effect of miR-326 on tumor volume and SMO suppression efficacy.

resultsSMO was upregulated in gliomas and was associated with tumor grade and survival period. SMO inhibition suppressed the biological behaviors of glioma cells. SMO expression was inversely correlated with miR-326 and was identified as a novel direct target of miR-326. miR-326 overexpression not only repressed SMO and downstream genes but also decreased the activity of the Hh pathway. Moreover, miR-326 overexpression decreased self-renewal and stemness and partially prompted differentiation in U251 tumor stem cells. In turn, the inhibition of Hh partially elevated miR-326 expression. Intracranial tumorigenicity induced by the transfection of miR-326 was reduced and was partially mediated by the decreased SMO expression.

conclusionsThis work suggests a possible molecular mechanism of the miR- 326/SMO axis, which can be a potential alternative therapeutic pathway for gliomas.

Indexed as

AnimalsBrain NeoplasmsCell Line, TumorGlioblastomaHedgehog ProteinsHumansKaplan-Meier EstimateMice, Inbred BALB CMice, NudeMicroRNAsNeoplastic Stem CellsReceptors, G-Protein-CoupledSignal TransductionSmoothened ReceptorSurvival RateUp-RegulationHedgehog ProteinsMicroRNAsMIRN326 microRNA, humanReceptors, G-Protein-CoupledSmoothened ReceptorSMO protein, humangliomahedgehogmiRNASMOstemness

Identifiers

PMID25173582
PMCPMC4288524
OpenAlexW2142877012

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.