ArticleThe Journal of physiology2014
Fluorescence recovery after photobleaching reveals regulation and distribution of connexin36 gap junction coupling within mouse islets of Langerhans.
Article in The Journal of physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Somatostatin receptors shape insulin and glucagon output within the pancreatic islet in mice through direct and paracrine effects.Diabetologia · 2026Article
- Diminished gap junction coupling under diabetogenic conditions does not drive loss of functional β-cell subpopulations.The Journal of general physiology · 2026Article
- Spatially bound functional heterogeneity drives modular organization in β-cell networks.Biophysical journal · 2025Article
- Review
- Islet architecture in adult mice is actively maintained by Robo2 expression in β cells.Developmental biology · 2024Article
- Article
- Restoring connexin-36 function in diabetogenic environments precludes mouse and human islet dysfunction.The Journal of physiology · 2023Article
- Disrupted glucose homeostasis and glucagon and insulin secretion defects in Robo βKO mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Light-stimulated insulin secretion from pancreatic islet-like organoids derived from human pluripotent stem cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Exendin-4 affects calcium signalling predominantly during activation and activity of beta cell networks in acute mouse pancreas tissue slices.Frontiers in endocrinology · 2023Article
- The effect of forskolin and the role of Epac2A during activation, activity, and deactivation of beta cell networks.Frontiers in endocrinology · 2023Article
- Review
- Liraglutide increases islet CaDiabetes, obesity & metabolism · 2022Article
- Functional architecture of pancreatic islets identifies a population of first responder cells that drive the first-phase calcium response.PLoS biology · 2022Article
- Dynamic changes in β-cell [CaMolecular metabolism · 2022Article
- Modulation of Gap Junction Coupling Within the Islet of Langerhans During the Development of Type 1 Diabetes.Frontiers in physiology · 2022Article
- From Isles of Königsberg to Islets of Langerhans: Examining the Function of the Endocrine Pancreas Through Network Science.Frontiers in endocrinology · 2022Review
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- The physiological role of β-cell heterogeneity in pancreatic islet function.Nature reviews. Endocrinology · 2022Review
- Reduced synchroneity of intra-islet CaeLife · 2021Article
Corrections and comments
- Erratum issuedCorrigenda.2015
Authors and funding
4 authors.
Funding
Abstract
The pancreatic islets are central to the maintenance of glucose homeostasis through insulin secretion. Glucose‐stimulated insulin secretion is tightly linked to electrical activity in β cells within the islet. Gap junctions, composed of connexin36 (Cx36), form intercellular channels between β cells, synchronizing electrical activity and insulin secretion. Loss of gap junction coupling leads to altered insulin secretion dynamics and disrupted glucose homeostasis. Gap junction coupling is known to be disrupted in mouse models of pre‐diabetes. Although approaches to measure gap junction coupling have been devised, they either lack cell specificity, suitable quantification of coupling or spatial resolution, or are invasive. The purpose of this study was to develop fluorescence recovery after photobleaching (FRAP) as a technique to accurately and robustly measure gap junction coupling in the islet. The cationic dye Rhodamine 123 was used with FRAP to quantify dye diffusion between islet β cells as a measure of Cx36 gap junction coupling. Measurements in islets with reduced Cx36 verified the accuracy of this technique in distinguishing between distinct levels of gap junction coupling. Analysis of individual cells revealed that the distribution of coupling across the islet is highly heterogeneous. Analysis of several modulators of gap junction coupling revealed glucose‐ and cAMP‐dependent modulation of gap junction coupling in islets. Finally, FRAP was used to determine cell population specific coupling, where no functional gap junction coupling was observed between α cells and β cells in the islet. The results of this study show FRAP to be a robust technique which provides the cellular resolution to quantify the distribution and regulation of Cx36 gap junction coupling in specific cell populations within the islet. Future studies utilizing this technique may elucidate the role of gap junction coupling in the progression of diabetes and identify mechanisms of gap junction regulation for potential therapies.
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