ArticleApplied and environmental microbiology2014
WblAch, a pivotal activator of natamycin biosynthesis and morphological differentiation in Streptomyces chattanoogensis L10, is positively regulated by AdpAch.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- A new paradigm for the regulation of A40926B0 biosynthesis.Synthetic and systems biotechnology · 2025Article
- Transcriptional regulators of secondary metabolite biosynthesis in Streptomyces.World journal of microbiology & biotechnology · 2024Review
- An intricate regulation of WblA controlling production of silent tylosin analogues and abolishment of expressible nikkomycin.Science China. Life sciences · 2023Article
- Genetic Engineering ofMicroorganisms · 2021Article
- Review
- WblA, a global regulator of antibiotic biosynthesis in Streptomyces.Journal of industrial microbiology & biotechnology · 2021Review
- Secondary nucleotide messenger c-di-GMP exerts a global control on natural product biosynthesis in streptomycetes.Nucleic acids research · 2020Article
- The Application of Regulatory Cascades inFrontiers in microbiology · 2020Review
- AdpAFrontiers in microbiology · 2019Article
- Regulation of antibiotic biosynthesis in actinomycetes: Perspectives and challenges.Synthetic and systems biotechnology · 2018Review
- The actinobacterial WhiB-like (Wbl) family of transcription factors.Molecular microbiology · 2018Review
- SbbR/SbbA, an Important ArpA/AfsA-Like System, Regulates Milbemycin Production inFrontiers in microbiology · 2018Article
- EngineeredFrontiers in microbiology · 2018Article
- Activation of a plasmid-situated type III PKS gene cluster by deletion of a wbl gene in deepsea-derived Streptomyces somaliensis SCSIO ZH66.Microbial cell factories · 2016Article
- Article
- Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes.Journal of industrial microbiology & biotechnology · 2016Review
- Biotechnological production and application of the antibiotic pimaricin: biosynthesis and its regulation.Applied microbiology and biotechnology · 2016Review
- Identification of novel tylosin analogues generated by a wblA disruption mutant of Streptomyces ansochromogenes.Microbial cell factories · 2015Article
- Activation of anthrachamycin biosynthesis in Streptomyces chattanoogensis L10 by site-directed mutagenesis of rpoB.Journal of Zhejiang University. Science. BArticle
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detailed mechanisms of WhiB-like (Wbl) proteins involved in antibiotic biosynthesis and morphological differentiation are poorly understood. Here, we characterize the role of WblAch, a Streptomyces chattanoogensis L10 protein belonging to this superfamily. Based on DNA microarray data and verified by real-time quantitative PCR (qRT-PCR), the expression of wblAch was shown to be positively regulated by AdpAch. Gel retardation assays and DNase I footprinting experiments showed that AdpAch has specific DNA-binding activity for the promoter region of wblAch. Gene disruption and genetic complementation revealed that WblAch acts in a positive manner to regulate natamycin production. When wblAch was overexpressed in the wild-type strain, the natamycin yield was increased by ∼30%. This provides a strategy to generate improved strains for natamycin production. Moreover, transcriptional analysis showed that the expression levels of whi genes (including whiA, whiB, whiH, and whiI) were severely depressed in the ΔwblAch mutant, suggesting that WblAch plays a part in morphological differentiation by influencing the expression of the whi genes.
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