ArticleThe Plant cell2014
Production of bioactive diterpenoids in the euphorbiaceae depends on evolutionarily conserved gene clusters.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 107 citations in OpenAlex.
- Molecular and metabolic drivers of oil quality and bioenergy potential in Jatropha curcas.Planta · 2026Article
- A Review of Plant-Derived Diterpenoid Biosynthesis: From Structural Scaffold Diversity and Lineage-Associated Distribution to Enzyme Mining and Discovery Strategies.Molecules (Basel, Switzerland) · 2026Review
- Heterologous Expression and In Vivo Functional Analysis of OsTPS2 in Yeast, Tobacco, and Rice.Plants (Basel, Switzerland) · 2026Article
- Ring contraction and ring expansion reactions in terpenoid biosynthesis and their application to total synthesis.Beilstein journal of organic chemistry · 2026Review
- Diterpenoids in medicinal plants: structure, distribution, biological activities, biosynthesis, and bioengineering prospects.Frontiers in plant science · 2026Review
- A new tool for engineering Phaeodactylum tricornutum: the METE promoter drives both high expression and BThe Plant journal : for cell and molecular biology · 2025Article
- Glycyrrhiza, a commonly used medicinal herb: Review of species classification, pharmacology, active ingredient biosynthesis, and synthetic biology.Journal of advanced research · 2025Review
- Identification of BAHD-acyltransferase enzymes involved in ingenane diterpenoid biosynthesis.The New phytologist · 2025Article
- Phytochemical Composition and Multifunctional Applications ofMolecules (Basel, Switzerland) · 2025Review
- Genome-wide terpene gene clusters analysis in Euphorbiaceae.Horticulture research · 2025Article
- MEANtools integrates multi-omics data to identify metabolites and predict biosynthetic pathways.PLoS biology · 2025Article
- Medicinal Plant and Traditional Knowledge-guided Strategies to Combat HIV Persistence.Current HIV/AIDS reports · 2024Review
- Genome-Wide Identification and Characterization of Diterpenoid Pathway CYPs inInternational journal of molecular sciences · 2024Article
- The Capsicum terpenoid biosynthetic module is affected by spider-mite herbivory.Plant molecular biology · 2023Article
- Hydroxylases involved in terpenoid biosynthesis: a review.Bioresources and bioprocessing · 2023Review
- An Engineered Heat-Inducible Expression System for the Production of Casbene inInternational journal of molecular sciences · 2023Article
- Plant terpene specialized metabolism: complex networks or simple linear pathways?The Plant journal : for cell and molecular biology · 2023Review
- Chromosome-level Genome Assembly of Euphorbia peplus, a Model System for Plant Latex, Reveals that Relative Lack of Ty3 Transposons Contributed to Its Small Genome Size.Genome biology and evolution · 2023Article
- Plant (di)terpenoid evolution: from pigments to hormones and beyond.Natural product reports · 2023Review
- Functional diversity and metabolic engineering of plant-specialized metabolites.Life metabolism · 2022Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Euphorbiaceae produce a diverse range of diterpenoids, many of which have pharmacological activities. These diterpenoids include ingenol mebutate, which is licensed for the treatment of a precancerous skin condition (actinic keratosis), and phorbol derivatives such as resiniferatoxin and prostratin, which are undergoing investigation for the treatment of severe pain and HIV, respectively. Despite the interest in these diterpenoids, their biosynthesis is poorly understood at present, with the only characterized step being the conversion of geranylgeranyl pyrophosphate into casbene. Here, we report a physical cluster of diterpenoid biosynthetic genes from castor (Ricinus communis), including casbene synthases and cytochrome P450s from the CYP726A subfamily. CYP726A14, CYP726A17, and CYP726A18 were able to catalyze 5-oxidation of casbene, a conserved oxidation step in the biosynthesis of this family of medicinally important diterpenoids. CYP726A16 catalyzed 7,8-epoxidation of 5-keto-casbene and CYP726A15 catalyzed 5-oxidation of neocembrene. Evidence of similar gene clustering was also found in two other Euphorbiaceae, including Euphorbia peplus, the source organism of ingenol mebutate. These results demonstrate conservation of gene clusters at the higher taxonomic level of the plant family and that this phenomenon could prove useful in further elucidating diterpenoid biosynthetic pathways.
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